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A. Hedley Brown, MS, FRCS; Nelson R. Niles, MD; Mark V. Braimbridge, FRCS; W. Gerald Austen, MD

AMA Arch Surg. 1972;105(4):622-627.

Abstract
Isolated canine hearts were used to evaluate retrograde gaseous oxygen perfusion of the heart as a method of preservation at room temperature for three hours compared with continuous normothermic cross-perfusion, ischemia at room temperature, and ischemia at 4 C, assessing the myocardium by functional, histochemical, and birefringence techniques. Statistically demonstrable benefit of oxygen insufflation over three hours of ischemia at the same temperature could be shown histochemically and there was also a trend toward functional benefit from this technique.

Author Affiliations
Boston

From the Department of Surgery, Harvard Medical School; and the General Surgical Services, Massachusetts General Hospital, Boston.

Footnotes
Accepted for publication June 28, 1972.

Reprint requests to Department of Surgery, Massachusetts General Hospital, Boston 02114 (Dr. Austen).

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