This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citing articles on HighWire  • Citing articles on Web of Science (38)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

I. In Vitro Multiplication of Rabbit-Skin Epithelial Cells

Aaron E. Freeman, PhD; Howard J. Igel, MD; Neil L. Waldman; Andrew M. Losikoff

AMA Arch Surg. 1974;108(5):721-723.

Abstract
In cases of extensive skin burns, there may not be sufficient viable skin to use as autografts. As a solution to this problem, we proposed that skin could be propagated in vitro, and the millions of cells thus grown could be used as autografts. The feasibility of this approach has been studied with rabbit skin. Under tissue culture conditions, small pieces of rabbit skin attach to supporting surfaces and proliferate to form layers of epithelial cells. A satisfactory supporting surface is the dermis of frozen porcine skin. With proper conditions, rabbit epithelial cell surface area can be expanded by a factor of 50 within 7 to 21 days.

Author Affiliations
Akron, Ohio

From the Children's Hospital, Akron, Ohio.

Footnotes
Accepted for publication Nov 21, 1973.

Reprint requests to Children's Hospital, Buchtel Ave at Bowery St, Akron, OH 44308 (Dr. Igel).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Cultured Skin Grafts: Past, Present, Future
Phillips
Arch Dermatol 1988;124:1035-1038.
ABSTRACT  

Porous Collagen Sponge Wound Dressings: in vivo and in vitro Studies
Doillon
J Biomater Appl 1987;2:562-578.
ABSTRACT  

Present Status of Plastic Surgery
Stephenson
JAMA 1975;234:929-929.
ABSTRACT