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Immune Cytolysis of Human Tumor Cells Mediated by Xenogeneic "Immune" RNA
Yosef H. Pilch, MD;
Larry L. Veltman, MD;
David H. Kern, PhD
AMA Arch Surg. 1974;109(1):30-34.
Abstract
Using a quantitative assay for lymphocyte-mediated cytotoxicity, it was shown that normal nonimmune human lymphocytes were converted to effector cells specifically cytotoxic to human tumor cells in vitro by incubation with "immune" RNA extracted from the lymphoid organs of specifically immunized guinea pigs or sheep. Lymphocytes incubated without RNA or with control RNA preparations failed to show cytotoxic activity. These immune responses were abrogated when the immune RNA was treated with RNAase prior to incubation with lymphocytes. However, treatment with DNAase or pronase did not alter the activity of the immune RNA.
Author Affiliations
Los Angeles
From the Department of Surgery, Harbor General Hospital, Torrance, Calif; the Tumor Immunology Laboratory, Veterans Administration Hospital, Sepulveda, Calif; and the UCLA School of Medicine, Los Angeles.
Footnotes
Accepted for publication Feb 19, 1974.
Read before the annual meeting of the Southern California Chapter of the American College of Surgeons, Palm Springs, Calif, Jan 18, 1974.
Reprint requests to Department of Surgery, Harbor General Hospital, 1000 W Carson St, Torrance, CA 90509 (Dr. Pilch).
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