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Assay in Colorectal Carcinoma Patients

Marvin S. Kaplan, MD; Frances O. Mino, MS; Kenneth B. Kummerfeld; Robert L. Lundak, PhD

Arch Surg. 1975;110(10):1217-1220.

Abstract
Tritiated thymidine incorporation by peripheral human lymphocytes stimulated with phytohemagglutinin (PHA) was investigated in 18 colorectal carcinoma patients (seven with localized tumor and 11 with metastatic tumors). The effect of sera obtained from these patients on lymphocytes from control patients was also studied.

The mean mitotic index of PHA-stimulated versus nonstimulated lymphocytes from all tumor patients was 29.2; from patients with localized tumors, 23.0; from patients with metastatic tumors, 26.0; and from controls, 29.4. In the presence of sera from tumor patients, the mitotic index in all tumor lymphocytes was 47.1; from localized tumor patients, 53.7; from metastatic tumor patients, 43.8; and from control lymphocytes, 47.4.

No substantial difference in mitotic index was detected in normal compared to tumor patient lymphocytes with or without normal or tumor serum.

Author Affiliations
From the Department of Surgery, University of California at Irvine (Drs. Kaplan and Lundak and Mr. Kummerfeld), and the Veterans Administration Hospital, Long Beach, Calif (Drs. Kaplan and Lundak, Ms. Mino, and Mr. Kummerfeld).

Footnotes
Accepted for publication Feb 24, 1975.

Read before the annual meeting of the Southern California Chapter of the American College of Surgeons, Santa Barbara, Calif, Jan 17, 1975.

Reprint requests to Department of Surgery, 5901 E Seventh St, Long Beach, CA 90801 (Dr. Kaplan).

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