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Early Experimental Studies With Cultured Autologous Canine Endothelium

Linda M. Graham, MD; David W. Vinter, MS; John W. Ford; Raymond H. Kahn, PhD; William E. Burkel, PhD; James C. Stanley, MD

Arch Surg. 1980;115(8):929-933.

Abstract
• Thirteen adult dogs underwent thoracoabdominal bypass operations with 6-mm, double-velour Dacron grafts 25 to 30 cm long. Experimental grafts were seeded with cultured autologous endothelial cells (n = 7). Unseeded grafts served as controls (n = 6). Endothelial cells were harvested from external jugular vein segments using 0.1% trypsin and 0.5% collagenase solutions. Grafts were studied at weeks 2 and 4. Endothelial cell coverage of experimental graft surfaces after two weeks was 60% to 70%, and approximately 80% after four weeks. Immunofluorescence using factor VIII-related antigen confirmed the graft's inner surface to be endothelium. Endothelial cell coverage in control grafts occurred as pannus ingrowth, and never exceeded more than 10% of the conduit surface. Generation of an early endothelial surface in prosthetic grafts is possible in a canine model using cultured autologous cells.

(Arch Surg 115:929-933, 1980)

Author Affiliations
From the Departments of Anatomy and Surgery, University of Michigan Medical School, Ann Arbor.

Footnotes
Accepted for publication March 7, 1980.

Reprint requests to University Hospital, 1405 E Ann St, Ann Arbor, MI 48109 (Dr Graham).

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