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Albert T. McManus, PhD; Charles G. McLeod, Jr, DVM; Arthur D. Mason, Jr, MD

Arch Surg. 1982;117(2):187-191.

Abstract
• We established a human burn isolate of Proteus mirabilis as an experimental pathogen. Infliction of a nonfatal scald injury (30%) rendered rats highly susceptible to lethal surface infection with this isolate. Dose-response experiments indicated that the lethal inoculation dose (50%) was less than 10³ organisms per square centimeter. Histopathologically, surface colonization was followed by progressive growth with subsequent invasion of viable tissue. The invasion was not characterized by the perivascular or perineural lesions observed in experimental Pseudomonas burn sepsis. Bacteriologic examinations showed moribund animals to be bacteremic with the test strain and to have wound biopsy counts exceeding 10⁶ organisms per gram of tissue. The role of bacterial motility as a virulence factor in this surface infection was investigated. Substrains selected for loss of subsurface spreading in soft agar lost virulence. This model of burn infection with a member of the Enterobacteriaceae should be used to evaluate topical and parenteral antimicrobial agents needed for the control of wound infections caused by such agents.

(Arch Surg 1982;117:187-191)

Author Affiliations
From the US Army Institute of Surgical Research, Fort Sam Houston, Tex. Dr McLeod is currently with the US Army Biomedical Laboratory, Aberdeen Proving Grounds, Md.

Footnotes
Accepted for publication July 27, 1981.

Read before the first annual meeting of the Surgical Infection Society, Chicago, April 25, 1981.

The views of the authors do not purport to reflect the positions of the Department of the Army or the Department of Defense.

Reprint requests to Library, US Army Institute of Surgical Research, Brooke Army Medical Center, Fort Sam Houston, TX 78234 (Dr McManus).

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