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Vol. 119 No. 1, January 1984 |
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PAPERS READ BEFORE THE THIRD ANNUAL MEETING OF THE SURGICAL INFECTION SOCIETY, FORT LAUDERDALE, FLA, MAY 7-10, 1983-PART I |
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Gas-Liquid Chromatography for Rapid Diagnosis of Intra-abdominal Infection
Carol A. Spiegel, PhD;
Mark A. Malangoni, MD;
Robert E. Condon, MD
Arch Surg. 1984;119(1):28-32.
Abstract
• We assessed the usefulness of gas-liquid chromatography in detecting fecal anaerobes in patients with suspected intra-abdominal infection related to the lower gastrointestinal tract. Twenty-five (89%) of 28 cultures with and five (26%) of 19 cultures without anaerobic isolates were positive for succinate. Data anlysis showed that Bacteroides but not Enterobacteriaceae organisms were responsible for succinate production. Volatile acids other than acetate (VAs) were present in 16 (57%) of 28 culture-positive and one (7%) of 14 culture-negative specimens. Sixteen (94%) of 17 VA-positive and seven (28%) of 25 VA-negative specimens had anaerobic isolates shown by culture. The presence of certain VAs was associated with the recovery of specific groups of anaerobic bacteria. The presence of succinate or VA in intra-abdominal fluid provides a specific, useful method for the rapid detection of fecal anaerobes in patients with intra-abdominal infections.
(Arch Surg 1984;119:28-32)
Author Affiliations
From the Department of Surgery, The Medical College of Wisconsin, Milwaukee.
Footnotes
Accepted for publication Aug 23, 1983.
Read before the Third Annual Meeting of the Surgical Infection Society, Fort Lauderdale, Fla, May 9, 1983.
Reprint requests to Research Service 151, Veterans Administration Medical Center, Wood, WI 53193 (Dr Spiegel).
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