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On Normal and Patient Polymorphonuclear Neutrophils With C3b-Coated Fluorescent Microspheres

James D. Ogle, PhD; Cora K. Ogle, PhD; J. Greg Noel, MS; Joseph S. Solomkin, MD; J. Wesley Alexander, MD, ScD

Arch Surg. 1985;120(1):104-109.

Abstract
• A method, devised in the authors' laboratories, for the determination of C3b receptors on normal and patient neutrophils using C3b-coated fluorescent microspheres, was applied to the quantitation of C3b receptors on the neutrophils of several patients suffering from burns and trauma and a patient with pancreatitis. From three to 11 days in the clinical course the relative number of C3b receptors was, or rose to, two to ten times the number of receptors present at later times in the clinical course and, in most of the cases studied, the increase in C3b receptor number coincided with enhanced neutrophil bactericidal function. The rise in C3b receptor number was ascribed to up-regulation by C5a and C5a des Arg from complement activation and also, in the cases where sepsis occurred, to the presence of bacterial chemotactic peptides. Preliminary experiments with zymosan-activated serum and the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine confirmed this explanation.

(Arch Surg 1985;120:104-109)

Author Affiliations
From the Departments of Biological Chemistry (Dr J. D. Ogle) and Surgery (Drs C. K. Ogle, Solomkin, and Alexander), University of Cincinnati Medical Center and the Shriners Burns Institute (Drs C. K. Ogle and Alexander and Mr Noel).

Footnotes
Accepted for publication Sept 12, 1984.

Read before the Fourth Annual Meeting of the Surgical Infection Society, Montreal, May 1, 1984.

Reprint requests to Department of Biological Chemistry, University of Cincinnati Medical Center, 231 Bethesda Ave, Cincinnati, OH 45267-0522 (Dr J. D. Ogle).

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