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Transplant Site Affects Splenic T- and B-Cell Survival and Function
Charles D. Livingston, MD;
Mary Pat Moyer, PhD;
Barry A. Levine, MD;
Kenneth R. Sirinek, MD, PhD
Arch Surg. 1985;120(1):89-92.
Abstract
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• We killed control animals and those with intraperitoneal and subcutaneous splenic autotransplants at intervals of up to one year to study gross, microscopic, and immunologic characteristics that might explain previously observed experimental variations in protection against postsplenectomy sepsis. Lymphocyte cell populations were studied qualitatively using concanavalin A (ConA)–and phytohemagglutinin—stimulated tritiated thymidine incorporation, and quantitatively by immunofluorescence assay. Subcutaneous implants showed fibrosis and lost mass, which was reflected in reduced numbers of functional B- and T-cell lymphocytes. Intraperitoneal implants grew during the observation period and demonstrated normal numbers of functional B- and T-cell lymphocytes. Previous experimental results showing protection against postsplenectomy sepsis following intraperitoneal but not subcutaneous autotransplantation may be the result of maintaining normal numbers of functional lymphocytes in the transplanted splenic tissue rather than a qualitative change in cellular function.
(Arch Surg 1985;120:89-92)
Author Affiliations
From the Department of Surgery, The University of Texas Health Science Center at San Antonio.
Footnotes
Accepted for publication Sept 24, 1984.
Read before the Fourth Annual Meeting of the Surgical Infection Society, Montreal, May 1, 1984.
Reprint requests to Department of Surgery, The University of Texas Health Science Center, 7703 Floyd Curl Dr, San Antonio, TX 78284 (Dr Sirinek).
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