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Harold Wenger, MD; Catherine Wong, MD; Robert H. Demling, MD

Arch Surg. 1985;120(2):159-165.

Abstract
• Our purpose was to determine whether peripheral soft tissues produce and release prostanoids in response to local sepsis, and whether this mediator release can produce pulmonary dysfunction. Escherichia coli endotoxin (2 µg/kg in 100 mL of saline) was injected below the hide of the flank in seven unanesthetized sheep. In three additional sheep, ibuprofen (12.5 mg/kg of body weight) was injected with the endotoxin. Thromboxane B₂ and 6-keto-PGF₁₀ (prostacyclin) levels were measured in tissue lymph draining the flank, lung lymph, pulmonary artery (Ppa), and aortic plasma. One hour after endotoxin administration, mean Pao₂ decreased from 90 to 74mm Hg and Ppa increased from 22 to 35mm Hg. Lung lymph flow (Q_L) increased only 50% with Q_L being protein poor. No increase in lung or peripheral soft-tissue vascular permeability was noted. Tissue lymph TxB₂) increased from 220±114 to greater than 10,000 pg/mL with levels in Ppa plasma increasing from 300±128 to 595±124 pg/mL and aortic plasma from 270 ± 141 to 410 ± 104 pg/mL. Lung lymph TxB₂ paralleled aortic values. Peak levels of 6-keto-PGF₁ in systemic lymph exceeded 2,000 pg/mL while levels in lung lymph remained relatively constant. The pulmonary injury and the increase in TxB₂ was prevented by ibuprofen. We conclude that the response of soft tissue to local endotoxin is to release thromboxane in quantities sufficient to raise plasma levels and to produce hypoxia and pulmonary hypertension. The lung dysfunction is not produced by an increase in lung water or vascular permeability.

(Arch Surg 1985;120:159-165)

Author Affiliations
From the Longwood Area Trauma Center, Harvard Medical School at Beth Israel and Brigham and Women's Hospitals and the Children's Hospital, Boston.

Footnotes
Accepted for publication Aug 15, 1984.

Read before the Fourth Annual Meeting of the Surgical Infection Society, Montreal, April 30, 1984.

Reprint requests to Longwood Area Trauma Center, 75 Francis St, Boston, MA 02115 (Dr Demling).

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ABSTRACT