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Differential Injury of Mitochondrial Subpopulations

Eric S. Weinstein, MD; Daniel W. Benson; Deborah J. Ratcliffe, MS; John Maksem, MD; Donald E. Fry, MD

Arch Surg. 1985;120(3):332-338.

Abstract
• Distinct populations of subsarcolemmal (SS) and interfibrillar (IF) rat cardiac mitochondria were studied following 15 and 30 minutes of warm and cold global ischemia. The respiratory control index, state 3, state 4, adenosine diphosphate—oxygen ratio, and specific enzyme activities of these mitochondrial populations were examined. The subsarcolemmar and IF mitochondria were both severely uncoupled and inhibited by warm ischemia. However, IF mitochondria had a higher RCI at each ischemic interval. In cold ischemia, IF mitochondria were not injured compared with control specimens. Subsarcolemmar mitochondria showed a trend towards a lower RCI that was statistically significant at 30 minutes with succinate as a substrate. These data implicate a differential injury of ischemia on the compartmentalized bioenergy metabolism of the myocardial cell.

(Arch Surg 1985;120:332-338)

Author Affiliations
From the Department of Surgery (Drs Weinstein and Fry, Mr Benson, and Ms Ratcliffe) and Pathology (Dr Maksem), Veterans Administration Medical Center, Cleveland; and the Department of Surgery, Case Western Reserve University, Cleveland (Drs Weinstein and Fry, Mr Benson, and Ms Ratcliffe). Dr Weinstein is a Dudley P. Allen Research Fellow for the Department of General Surgery at Case Western Reserve University Hospitals.

Footnotes
Accepted for publication Aug 29, 1984.

Read before the Eighth Annual Surgical Symposium of the Association of Veterans Administration Surgeons, Los Angeles, May 11, 1984.

Reprint requests to Chief, Surgical Service (112W), VA Medical Center, 10701 East Blvd, Cleveland, OH 44106 (Dr Fry).

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