This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

A Single Murine Monoclonal Antibody Provides Cross-Genera Protection

David L. Dunn, MD, PhD; David C. Ewald; Naresh Chandan, MS; Frank B. Cerra, MD

Arch Surg. 1986;121(1):58-62.

Abstract
• Previous experimental and clinical studies have demonstrated the ability of polyclonal antibody directed against the core lipopolysaccharide (LPS)-lipid A component of endotoxin to reduce mortality. We sought to characterize the ability of a single murine monoclonal IgG, antibody (8A1 MAb) to react to a variety of gram-negative microorganisms, to promote phagocytosis, and to provide protection during experimental murine sepsis. The 8A1 MAb reacted to various gram-negative bacterial whole cell and LPS antigens examined by enzyme-linked immunosorbent assay. Reactivity was highest to Salmonella minnesota Re LPS and lipid A. Phagocytosis was promoted by this monoclonal antibody to several gram-negative bacteria, except Pseudomonas aeruginosa. The 8A1 MAb (2 mg per mouse) enhanced survival during bacteremia due to either Escherichia coli 0111:B4 or Klebsiella pneumoniae, and during endotoxemia due to all types of LPS examined except P aeruginosa. We concluded that a single MAb with anti—lipid A specificity was cross reactive in vitro and cross protective in vivo. A clinical trial comparing polyclonal and monoclonal antibody in high-risk septic patients seems warranted.

(Arch Surg 1986;121:58-62)

Author Affiliations
From the Department of Surgery, University of Minnesota, Minneapolis.

Footnotes
Accepted for publication Oct 1, 1985.

Read before the Fifth Annual Meeting of the Surgical Infection Society, New Orleans, April 29, 1985.

Reprint requests to Box 242 Mayo Memorial Bldg, 420 Delaware St SE, University of Minnesota, Minneapolis, MN 55455 (Dr Dunn).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Review: Evidence against the hypothesis that antibodies to the inner core of lipopolysaccharides in antisera raised by immunization with enterobacterial deep-rough mutants confer broad-spectrum protection during Gram-negative bacterial sepsis
Greisman and Johnston
Innate Immunity 1997;4:123-153.
ABSTRACT  

Decreased Tumor Necrosis Factor Production During the Initial Stages of Infection Correlates With Survival During Murine Gram-negative Sepsis
Mayoral et al.
Arch Surg 1990;125:24-28.
ABSTRACT  

Protective Capacity of Polyclonal and Monoclonal Antibodies Directed Against Endotoxin During Experimental Sepsis
Dunn et al.
Arch Surg 1988;123:1389-1393.
ABSTRACT  

Posttraumatic Multisystem Organ Failure
DeCamp and Demling
JAMA 1988;260:530-534.
ABSTRACT