You are seeing this message because your Web browser does not support basic Web standards. Find out more about why this message is appearing and what you can do to make your experience on this site better.


ABOUT ARCHIVES
Advanced Search

Welcome   | My Account | E-mail Alerts | Access Rights | Sign In


  Vol. 121 No. 2, February 1986 TABLE OF CONTENTS
  Archives
  •  Online Features
  PAPERS READ BEFORE THE FIFTH ANNUAL MEETING OF THE SURGICAL INFECTION SOCIETY, NEW ORLEANS, APRIL 29 TO APRIL 30, 1985-PART II
 This Article
 •References
 •Full text PDF
 •Send to a friend
 • Save in My Folder
 •Save to citation manager
 •Permissions
 Citing Articles
 •Citation map
 •Citing articles on HighWire
 •Contact me when this article is cited
 Related Content
 •Similar articles in this journal
 Social Bookmarking
  Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit Add to Technorati Add to Twitter What's this?

Trauma Serum Suppresses Superoxide Production by Normal Neutrophils

Marc E. Lanser, MD; Glenn E. Brown, PhD; Rafael Mora, MD; William Coleman, PhD; John H. Siegel, MD

Arch Surg. 1986;121(2):157-162.


Abstract

• The effect of trauma serum on Superoxide production by normal neutrophils was studied in 47 serum samples from 18 patients with multiple trauma. Ten patients became septic and eight patients remained nonseptic. Incubation in trauma serum significantly suppressed Superoxide production by normal neutrophils compared with incubation in normal serum: 3.6±1.44 vs 4.04±1.64 nmole of Superoxide produced by 106 neutrophils (mean±SD). There was no difference in the suppressive effect between septic and nonseptic trauma serum samples. The chemlluminescence response of normal neutrophils was likewise suppressed following incubation in trauma serum compared with incubation in normal serum. The chemiluminescence response correlated with Superoxide reduction of cytochrome C. In addition, the chemiluminescence response was significantly less in septic-trauma serum than in nonseptic-trauma serum. Suppressive serum was found to inhibit the neutrophil-membrane depolarization response to latex particles, as measured by flow cytometry. We conclude that trauma serum suppresses Superoxide production by normal neutrophils, and that such suppression can be detected reliably using the clinically applicable technique of chemiluminescence. A normal chemiluminescence response excludes serum-mediated suppression of neutrophil Superoxide production. In addition, chemiluminescence may be of value in detecting altered resistance to sepsis following injury, while Superoxide determinations do not seem to be helpful in this regard. The mechanism of action of the suppressor may involve reversible inhibition of membrane depolarization necessary for the production of bactericidal oxygen species.

(Arch Surg 1986;121:157-162)



Author Affiliations

From the Department of Surgery, Maryland Institute for Emergency Medical Services, Baltimore (Drs Lanser, Mora, Coleman, and Siegel); and the University of Maryland Cancer Center, Baltimore (Dr Brown).


Footnotes

Accepted for publication Oct 31, 1985.

Read before the Fifth Annual Meeting of the Surgical Infection Society, New Orleans, April 29, 1985.

Reprint requests to Department of Surgery, Beth Israel Hospital, 330 Brookline Ave, Boston, MA 02215 (Dr Lanser).



Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Complement-Induced Impairment of Innate Immunity During Sepsis
Huber-Lang et al.
J. Immunol. 2002;169:3223-3231.
ABSTRACT | FULL TEXT  

Leucocytes and cardiopulmonary bypass: in vitro production of oxygen free radicals and trapping in the reperfused myocardium
Semb et al.
Perfusion 1990;5:169-180.
ABSTRACT  

Intravenous Fat Emulsion Acutely Suppresses Neutrophil Chemiluminescence
Robin et al.
JPEN J Parenter Enteral Nutr 1989;13:608-613.
ABSTRACT  

Granulocyte Chemiluminescence in Patients With Postoperative Infections
Salo et al.
Arch Surg 1988;123:17-22.
ABSTRACT  





HOME | CURRENT ISSUE | PAST ISSUES | TOPIC COLLECTIONS | CME | SUBMIT | SUBSCRIBE | HELP
CONDITIONS OF USE | PRIVACY POLICY | CONTACT US | SITE MAP
 
© 1986 American Medical Association. All Rights Reserved.