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Jonathan E. Hasson, MD; Deborah H. Wiebe, MS; William M. Abbott, MD

Arch Surg. 1987;122(4):428-430.

Abstract
• We studied the ability of polymers from which vascular prostheses (VPs) are or could be fabricated to support attachment and migration of adult human vascular endothelial cells (AHVECs) in an in vitro system. Polymers included Mylar, Teflon, two novel bioabsorbable polymers with different biologic half-lives (BR1 and BR2), and an elastic nonabsorbable biocompatible polymer (EBN). Both BR1, BR2, and EBN supported AHVEC attachment well (56%, 62%, and 71%, respectively, of plated cells at one hour, respectively) but differed in their ability to support AHVEC migration. The AHVECs attached less well to Mylar and Teflon (39% and 21%), and in no case were AHVECs observed to grow or migrate on either of these polymers. This finding correlates with the clinical observation that VPs fabricated from Mylar and Teflon uniformly fail to develop an endothelial cell lining after implantation in vivo. Future VPs, fabricated from materials chosen for their ability to support AHVEC attachment, growth, and migration in vitro, might better support a spontaneous endothelial cell lining after implantation in humans.

(Arch Surg 1987;122:428-430)

Author Affiliations
From the Cell Culture Division, Vascular Research Laboratory, Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston.

Footnotes
Accepted for publication Dec 17, 1986.

Read before the 67th Annual Meeting of the New England Surgical Society, Dixville Notch, NH, Sept 26, 1986.

Reprint requests to Cell Culture Division, Vascular Research Laboratory, Massachusetts General Hospital, White 3-A, Fruit Street, Boston, MA 02114 (Dr Hasson).

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