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Monoclonal Antibody—Mediated Modulation of Parathyroid Hormone Secretion by Dispersed Parathyroid Cells
James T. Posillico, PhD;
Richard E. Wilson, MD;
Sri S. Srikanta, MD;
George S. Eisenbarth, MD, PhD;
Michelle Letarte, PhD;
Elizabeth J. Quackenbush, PhD;
Vito Quaranta, MD;
Shana Kajaji, PhD;
Edward M. Brown, MD
Arch Surg. 1987;122(4):436-442.
Abstract
• Available data suggest that ionized calcium may interact with a cell surface "sensor" or "receptor" to produce changes in one or more intracellular second messengers that ultimately regulate the release of parathyroid hormone (PTH). Recently, we developed a series of monoclonal antibodies directed toward specialized differentiation antigens expressed on endocrine cells. Since many of these monoclonal antibodies displayed exquisite specificity for cell surface molecules on the parathyroid cell, we used these reagents as probes to investigate signal recognition/transduction mechanisms associated with abnormal calcium-regulated PTH secretion. Depending on their binding site on the respective target antigen molecules, these monoclonal antibodies either stimulated or inhibited hormone secretion. Thus, defects in membraneassociated structures may contribute to deranged calciumregulated PTH secretion in abnormal parathyroid cells.
(Arch Surg 1987;122:436-442)
Author Affiliations
From the Departments of Medicine (Drs Posillico and Brown) and Surgery (Dr Wilson), Brigham and Women's Hospital and Harvard Medical School, Boston; Department of Medicine, Joslin Diabetes Center and Harvard Medical School, Boston (Drs Srikanta and Eisenbarth); Department of Immunology, The Hospital for Sick Children, Toronto (Drs Letarte and Quackenbush); and Department of Immunology, Scripps Clinic and Research Foundation, La Jolla, Calif (Drs Quaranta and Kajaji).
Footnotes
Accepted for publication Dec 30, 1986.
Read before the 67th Annual Meeting of the New England Surgical Society, Dixville Notch, NH, Sept 27, 1986.
Reprint requests to Endocrine-Hypertension Unit, Department of Medicine, Brigham and Women's Hospital, 75 Francis St, Boston, MA 02115 (Dr Posillico).
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