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Philip B. Paty, MD; Ronald W. Graeff, MS; Frederic M. Waldman, MD, PhD; Thomas K. Hunt, MD; Stephen J. Mathes, MD

Arch Surg. 1988;123(12):1509-1513.

Abstract
• Migration of neutrophils from blood into tissue is a complex response by circulating cells to chemotactic stimulation. Previous studies of the functional changes induced by this process have produced variable results. We compared neutrophils isolated from blood and from subcutaneous wounds in rabbits using established assays for adherence, chemotaxis, superoxide anion production, and hydrogen peroxide production. No differences in adherence to biologic surfaces or chemotaxis toward activated plasma were found. However, our results confirm the observation that wound neutrophils are "primed" for increased production of oxygen radicals. Primed responses were observed for both soluble (formyl methionyl leucylphenylalanine, phorbol myristate acetate) and particulate (opsonized zymosan) stimulants. Priming was also observed for peritoneal exudate neutrophils. The data suggest that the process of extravascular migration includes priming of the superoxide generating system.

(Arch Surg 1988;123:1509-1513)

Author Affiliations
From the Departments of Surgery (Drs Paty, Hunt, and Mathes, and Mr Graeff) and Laboratory Medicine (Dr Waldman), University of California, San Francisco.

Footnotes
Accepted for publication June 23, 1988.

Read before the Eighth Annual Meeting of the Surgical Infection Society, San Francisco, May 6, 1988.

Reprint requests to Department of Surgery, University of California, San Francisco, 350 Parnassus, #509, San Francisco, CA 94122 (Dr Mathes).

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