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Rabie N. Stephan, MD; John M. Kisala, MD; Richard E. Dean, MD; Alexander S. Geha, MD; Irshad H. Chaudry, PhD

Arch Surg. 1988;123(2):235-240.

Abstract
• To study the effect of blood transfusion (BT) on cell-mediated immunity, we examined the antigen presentation function of peritoneal macrophages and interleukin 2 (IL-2) generation by splenocytes. C3H/HEJ mice were transfused with 0.2 mL of fresh allogeneic blood obtained from C57BL/6 mice; they were killed on days 1, 3, and 7 after BT. A second group of C3H/HEJ mice was transfused with 0.2 mL/d of the same allogeneic blood on three successive days; they were killed on day 7 following the last BT. The antigen presentation function of peritoneal macrophages was measured by utilizing a D10.G4.1 T-helper cell clone; IL-2 activity in supernatants of concanavalin A—stimulated splenocytes was tested by utilizing an IL-2-dependent HT-2 cell line. The results indicate that although antigen presentation function remains unaffected after single and multiple BTs, the ability of splenocytes to generate IL-2 decreases significantly even after a single BT. Thus, the increased susceptibility to infection and the additional immune perturbations in malignant neoplasms following BT may be due in part to decreased IL-2 generation.

(Arch Surg 1988;123:235-240)

Author Affiliations
From the Departments of Surgery (Drs Stephan, Kisala, Dean, and Chaudry) and Physiology (Dr Chaudry), Michigan State University, East Lansing; and the Department of Surgery, Case Western Reserve University, Cleveland (Dr Geha).

Footnotes
Accepted for publication Sept 18, 1987.

Read before the Seventh Annual Meeting of the Surgical Infection Society, Philadelphia, May 12, 1987.

Reprint requests to Department of Surgery, Michigan State University, B424 Clinical Center, East Lansing, MI 48824-1315 (Dr Chaudry).

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