This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Citing articles on Web of Science (41)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

Robert A. Kozol, MD; Concettina Gillies, MS; Salwa A. Elgebaly, PhD

Arch Surg. 1988;123(4):420-423.

Abstract
• This study investigates the effects of sodium hypochlorite, or Dakin's solution (DS), on the function and viability of cells of the wound module (neutrophils, fibroblasts, and endothelial cells). For functional studies, the influence of DS on the in vitro migration of neutrophils was evaluated. Our data indicate that DS (2.5x10²% to 2.5x10⁴%) results in greater than 90% inhibition of the migration of both stimulated and nonstimulated neutrophils. Electron microscopy and trypan blue evaluation of neutrophils exposed to DS at these concentrations revealed normal structural features, which indicates that the observed reduction in neutrophil migration is not a result of cell death. In contrast to neutrophils, cultured fibroblasts and endothelial cells exposed to DS (2.5x10²% or 2.5x10³%) for 30 minutes show marked cell injury characterized by convoluted nuclei, cytoplasmic vacuolization, dilated endoplasmic reticulum, and swollen mitochondria on electron microscopy. These data suggest that DS, even at very dilute concentrations, is toxic to cells of the wound module. We therefore recommend abandonment of the use of DS in open wounds.

(Arch Surg 1988;123:420-423)

Author Affiliations
From the Departments of Surgery (Drs Kozol and Elgebaly) and Pathology (Ms Gillies), University of Connecticut School of Medicine, Farmington.

Footnotes
Accepted for publication Sept 24, 1987.

Read before the Association of Veterans Administration Surgeons, Portland, Ore, May 8, 1987.

Reprint requests to Department of Surgery, University of Connecticut School of Medicine, Farmington, CT 06032 (Dr Kozol).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

A Critical Discussion of the Use of Antiseptics in Acute Traumatic Wounds
Smith
J. Am. Podiatr. Med. Assoc. 2005;95:148-153.
ABSTRACT | FULL TEXT  

Effects of Topical Antimicrobial Agents on the Human Neutrophil Respiratory Burst
Hansbrough et al.
Arch Surg 1991;126:603-608.
ABSTRACT  

Neutrophil Chemotactic Activity in Human Gastric Secretions
Kozol et al.
Arch Surg 1990;125:454-456.
ABSTRACT  

Debridement of Wounds With Dakin's Solution
RAFFENSPERGER
Arch Surg 1989;124:133-133.
ABSTRACT  

Effects of Sodium Hypochlorite (Dakin's Solution) on Wound Cells
FULLER
Arch Surg 1988;123:1526-1526.
ABSTRACT  

Dakin's Solution in Wound Treatment
ALLAN
Arch Surg 1988;123:1525-1526.
ABSTRACT  

Tissue Injury Caused by Dakin's Solution
LINEAWEAVER
Arch Surg 1988;123:1286-1286.
ABSTRACT