This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

Response to Leucine and -Ketoisocaproic Acid

Per-Olof Hasselgren, MD, PhD; J. Howard James; Brad W. Warner, MD; Robert P. Hummel, III; Josef E. Fischer, MD

Arch Surg. 1988;123(5):640-644.

Abstract
• It has been suggested that leucine and -ketoisocaproic acid (KIA) stimulate protein synthesis and reduce protein breakdown and may be useful in the treatment of muscle catabolism during sepsis. However, whether leucine and KIA regulate protein turnover in septic skeletal muscle is not known. In this study, intact muscles from untreated normal rats or from rats subjected to cecal ligation and puncture were incubated in the presence of leucine or KIA. In normal muscle, leucine stimulated protein synthesis and reduced protein degradation, while KIA decreased protein breakdown. In septic muscle, protein synthesis was also stimulated by leucine, but only at a concentration higher than that needed to affect protein synthesis in normal muscle. Protein breakdown in septic muscle was unaffected by leucine and KIA even at an extracellular concentration as high as 5 mmol/L. Since other experiments showed that the intracellular concentration of leucine was not different in incubated normal and septic muscles, these results suggest that sepsis induces changes in skeletal muscle protein turnover that are resistant to the effects of leucine.

(Arch Surg 1988;123:640-644)

Author Affiliations
From the Department of Surgery, University of Cincinnati Medical Center.

Footnotes
Accepted for publication April 21, 1987.

Reprint requests to Department of Surgery, University of Cincinnati Medical Center, 231 Bethesda Ave, Cincinnati, OH 45267-0558 (Dr Fischer).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Therapeutic Use of Branched-Chain Amino Acids in Burn, Trauma, and Sepsis
De Bandt and Cynober
J. Nutr. 2006;136:308S-313S.
ABSTRACT | FULL TEXT  

Dietary Amino Acids Promote Pancreatic Protease Synthesis at the Translation Stage in Rats
Hashimoto and Hara
J. Nutr. 2003;133:3052-3057.
ABSTRACT | FULL TEXT  

The Anabolic Effects of IGF-1 in Skeletal Muscle After Burn Injury Are not Caused by Increased Cell Volume
Fang et al.
JPEN J Parenter Enteral Nutr 1998;22:115-119.
ABSTRACT  

Is Muscle Protein Turnover Regulated by Intracellular Glutamine During Sepsis?
Fang et al.
JPEN J Parenter Enteral Nutr 1995;19:279-285.
ABSTRACT