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Abnormal Rabbit Heterophil Chemotaxis Following Thermal InjuryAn In Vivo Model of an Abnormality of the Chemoattractant Receptor for f-met-leu-phe
John Mihran Davis, MD;
John I. Gallin, MD
Arch Surg. 1988;123(6):752-755.
Abstract
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• Previous studies have shown that the decreased neutrophil migratory responsiveness seen in burned patients correlates with the extent of thermal injury and the extent of the neutrophil-specific granule deficiency. To understand better the relationship between the neutrophil dysfunction, degranulation, and thermal injury, a rabbit model was studied. Eighteen rabbits were burned over 20% of their surface area. Assay of peripheral blood heterophils disclosed decreased migratory activity compared with preburn levels and decreased lysozyme content vs preburn levels, but no change in the β-glucuronidase content. The specific binding of tritiated formyl-methionyl-leucyl-phenylalanine to peripheral blood heterophils was increased fivefold over that of control cells. These studies indicate that, following thermal injury, there is a selective decrease of specific granule contents and an increase in chemoattractant binding to the cell and also suggest an abnormality in chemoattractant receptor processing. The rabbit provides a convenient model for the study of compromised host defenses following thermal injury.
(Arch Surg 1988;123:752-755)
Author Affiliations
From the Department of Surgery, Cornell University Medical College, New York (Dr Davis); and the Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Washington, DC (Dr Gallin).
Footnotes
Accepted for publication March 27, 1987.
Reprint requests to 1300 York Ave, New York, NY 10021 (Dr Davis).
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