 |
|
Flow Cytometric Measurements of Nuclear DNA and Ploidy Analysis in Hürthle Cell Neoplasms of the Thyroid
Michael K. McLeod, MD;
Norman W. Thompson, MD;
Jerry L. Hudson, PhD;
Jacqueline A. Gaglio;
Ricardo V. Lloyd, MD, PhD;
Jay K. Harness, MD;
Ronald Nishiyama, MD;
Polly S. Y. Cheung, MD
Arch Surg. 1988;123(7):849-854.
Abstract
| |
• Nuclear DNA content and nuclear DNA ploidy were measured In 36 Hürthle cell neoplasms (HCNs) to better define their potential roles in predicting the clinical behavior of these lesions. An EPICS V flow cytometer (Coulter Electronics, Hialeah, Fla) was used. Measurements were taken from paraffin-embedded tissue. Isolated nuclei were stained with propidium iodide. The study was conducted in a blinded fashion. Observed NDC and PDY patterns were classified as diploid, aneuploid, or suspicious. Twenty-nine lesions (81%) were diploid and seven (19%) were aneuploid. Twelve (33%) HCNs were malignant, 23 (64%) were benign, and one (3%) was Indeterminate. Eight (67%) of 12 malignant HCNs were diploid and four (33%) of 12 were aneuploid. In comparison, 20 (87%) of 23 benign lesions were diploid and three (13%) of 23 were aneuploid. The indeterminate neoplasm was diploid. There were three deaths in this group of patients; all three had aneuploid neoplasms, and all had locally recurrent disease with distant metastases. There was a significant cross correlation between histologic features and DNA content with regard to outcome. These preliminary data suggest that NDC and PDY are not helpful in distinguishing histologically benign from malignant HCNs; however, they may be useful in determining prognosis.
(Arch Surg 1988;123:849-854)
Author Affiliations
From the Departments of Surgery (Drs McLeod, Thompson, and Harness) and Pathology (Drs Hudson, Gaglio, and Lloyd), University of Michigan Medical Center, Ann Arbor; the Department of Pathology, Maine Medical Center, Portland (Dr Nishiyama); and the Department of Surgery, University of Hong Kong, Queen Mary Hospital (Dr Cheung).
Footnotes
Accepted for publication Feb 10, 1988.
Read before the 95th Annual Meeting of the Western Surgical Association, Dallas, Nov 17, 1987.
Reprint requests to Taubman Health Care Center, Room 2920F, 1500 E Medical Center Dr, Ann Arbor, MI 48109-0331 (Dr McLeod).
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter
What's this?
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
My approach to oncocytic tumours of the thyroid
Asa
J. Clin. Pathol. 2004;57:225-232.
ABSTRACT
| FULL TEXT
|
