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John V. Reynolds, FRCS(I); Jian Shou, MD; Hoon Choi, MD; Robert Sigal, MD; Moritz M. Ziegler, MD; John M. Daly, MD

Arch Surg. 1989;124(2):235-239.

Abstract
• Human neuroblastoma (NRB) cell lines are markedly sensitive to natural killer (NK) cell lysis in vitro, but patients with NRBs have low or absent NK activity. This study evaluated the NK sensitivity of murine NRBs (C1300 and TBJ) in the regulation of NRB growth and determined the effects of recombinant (r) interferon gamma and recombinant interleukin 2 (rIL-2). Both basal (8%±3% specific cytotoxicity) and induced (20%±3%) NK lyses of C1300-NRB were observed. In vivo depletion of NK cells with anti–asialo GM-1 significantly enhanced growth of C1300-NRB and deceased survival. Treatment wth r–interferon gamma or rIL-2 on days 1 through 3 after C1300-NRB inoculation significantly prolonged the mean tumor latency period, decreased the tumor growth rate, and enhanced in vitro NK killing of C1300-NRB and YAC-1. The effects of R–interferon gamma and IL-2 were abrogated by pretreatment with anti–asialo GM-1. These results demonstrated that NK cells form one important component of regulation of a murine NRB, but immunomodulation with potent lymphokines requires cooperation of more than one cell type.

(Arch Surg 1989;124:235-239)

Author Affiliations
From the Departments of Surgery, The Harrison Department of Surgical Research (Drs Reynolds, Shou, Sigal, and Daly) and the University of Pennsylvania School of Medicine, Philadelphia, and the Joseph Stokes, Jr, Research Institute, The Children's Hospital of Philadelphia (Drs Choi and Ziegler).

Footnotes
Accepted for publication Oct 12, 1988.

Read before the Annual Meeting of the Society of Surgical Oncology, New Orleans, May 24, 1988.

Reprint requests to Division of Surgical Oncology, Department of Surgery, Hospital of the University of Pennsylvania, 3400 Spruce St, Philadelphia, PA 19104 (Dr Daly).

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