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Howard P. Greisler, MD; Eric D. Endean, MD; John J. Klosak, MD; Joan Ellinger; Scott C. Henderson; Si M. Pham, MD; Steven J. Durham, MD; David P. Showalter, MD; Jeffery Levine, PhD; Harvey S. Borovetz, PhD

Arch Surg. 1989;124(4):429-433.

Abstract
• The establishment of an early blood-contacting endothelialized surface may improve the graft-host relationship. This study evaluated the adherence of indium 111-radiolabeled endothelial cells that were cultured to confluence on fibronectin-treated polyester elastomer (Hytrel) grafts that were perfused for two hours on a pulse duplicator apparatus under high- and low-shear conditions. Perfusate samples were serially assayed for radioactivity. After perfusion, grafts were sectioned into four segments and assayed for retained radioactivity. All graft segments were hematoxylin stained and examined under light microscopy for evaluation of cell density. Excellent endothelial cell adherence (90%) was observed under both hemodynamic conditions at 120 minutes, with most losses occurring within the first 15 minutes. No differences were seen between high- and low-shear conditions or proximal vs distal graft segments.

(Arch Surg 1989;124:429-433)

Author Affiliations
From the Department of Surgery, Loyola University Medical Center, Maywood, Ill (Drs Greisler, Endean, and Klosak); the Department of Research, Hines (Ill) Veterans Administration Hospital (Dr Greisler, Ms Ellinger, and Mr Henderson); and the Departments of Surgery (Drs Pham, Durham, Showalter, and Borovetz) and Nuclear Medicine (Dr Levine), University of Pittsburgh School of Medicine.

Footnotes
Accepted for publication Dec 6, 1988.

Read before the 12th Annual Surgical Symposium of the Association of Veterans Administration Surgeons, Minneapolis, May 14, 1988.

Reprint requests to Loyola University Medical Center, 2160 S First Ave, Maywood, IL 60153 (Dr Greisler).

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