 |
|
Decreased Tumor Necrosis Factor Production During the Initial Stages of Infection Correlates With Survival During Murine Gram-negative Sepsis
Jaime L. Mayoral, MD;
CyrilJ. Schweich;
David L. Dunn, MD, PhD
Arch Surg. 1990;125(1):24-28.
Abstract
| |
• Secretion of tumor necrosis factor (TNF)/cachectin occurs during gram-negative bacterial sepsis in response to macro-phage stimulation by lipopolysaccharide (endotoxin) and may play an early pivotal role in the subsequent host response. We sought to determine whether administration of: (1) murine monoclonal antibody directed against endotoxin, (2) steroids, or (3) antimicrobial agents would abrogate TNF production and whether the protective capacity would correlate with TNF levels in an experimental model of murine gram-negative bacterial sepsis. Mice were pretreated with anti–lipopolysaccharide monoclonal antibody, gentamicin sulfate, hydrocortisone, or saline and were then challenged with a lethal dose of intraperitoneal Salmonella minnesota. Murine serum TNF levels were measured by the L929 fibroblast cytotoxicity assay. Both gentamicin and anti–lipopolysaccharide monoclonal antibody significantly enhanced survival, and TNF activity at 1.5 and 3 hours was significantly suppressed in animals receiving these agents compared with animals that received either steroids or saline. We conclude that agents such as gentamicin, which inhibits bacterial replication, or monoclonal antibodies, which may neutralize lipopolysaccharide, indeed enhance survival, and survival was correlated with a significant reduction in circulating TNF during the early stages of infection.
(Arch Surg. 1990;125:24-28)
Author Affiliations
From the Division of Surgical Infectious Diseases, Department of Surgery, University of Minnesota, Minneapolis.
Footnotes
Accepted for publication September 12, 1989.
Read before the Ninth Annual Meeting of the Surgical Infection Society, Denver, Colo, April 13, 1989.
Reprints not available.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter
What's this?
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
Bovine Intestinal Alkaline Phosphatase Attenuates the Inflammatory Response in Secondary Peritonitis in Mice
van Veen et al.
Infect. Immun. 2005;73:4309-4314.
ABSTRACT
| FULL TEXT
Detrimental Effects of a Nitric Oxide Synthase Inhibitor (N-{omega}-Nitro-L-Arginine-Methyl-Ester) in a Murine Sepsis Model
Fukatsu et al.
Arch Surg 1995;130:410-414.
ABSTRACT
Inhibition of Splenic Macrophage Tumor Necrosis Factor {alpha} Secretion In Vivo by Antilipopolysaccharide Monoclonal Antibodies
Battafarano et al.
Arch Surg 1994;129:179-186.
ABSTRACT
Anti--Endotoxin Monoclonal Antibodies Protect by Enhancing Bacterial and Endotoxin Clearance
Burd et al.
Arch Surg 1993;128:145-151.
ABSTRACT
Anti--Tumor Necrosis Factor Antibody Reduces Mortality in the Presence of Antibiotic-Induced Tumor Necrosis Factor Release
Sawyer et al.
Arch Surg 1993;128:73-78.
ABSTRACT
|
