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Howard P. Greisler, MD; Steven Johnson, MD; Kathleen Joyce, PhD; Scott Henderson; Narendra M. Patel; Taha Alkhamis, PhD; Richard Beissinger, PhD; Dae Un Kim, MD

Arch Surg. 1990;125(12):1622-1625.

Abstract
• We evaluated the adherence of indium 111–radiolabeled endothelial cells to fibronectin-treated expanded polytetrafluoroethylene surfaces exposed to high (437 s^–1) vs low (218 s^–1) shear and the influence of shear on prostacyclin production. Canine jugular vein factor VIII–positive endothelial cells in passages 3 through 6 were incubated with¹¹¹Indium-oxine, and labeled cells were seeded onto fibronectin-treated expanded polytetrafluoroethylene patches. Patches with confluent cells were exposed to shear in a Weissenberg rheogoniometer for intervals ranging up to 60 minutes. Percent endothelial cell retention was determined by gamma counting of patches and media and by histologic evaluation. Prostacyclin production (tritiated radioimmunoassay of 6-keto-prostaglandin F₁) was assayed on perfusing media. Results showed no differences in 6-keto-prostaglandin F₁ production between shear rates or time periods. Endothelial cell retention did not differ between the shear rates. Rotational shear caused persistent cell loss over time in either high- or low-shear conditions. This persistent cell loss in response to steady rotational shear differs from that in response to identical rates of pulsatile linear shear in our laboratory where cell loss approached zero after 15 minutes.

(Arch Surg. 1990;125:1622-1625)

Author Affiliations
From the Department of Surgery, Loyola University Medical Center, Maywood, Ill (Drs Greisler, Johnson, and Joyce and Messrs Henderson and Patel); the Department of Chemical Engineering, Illinois Institute of Technology, Chicago (Drs Alkhamis and Beissinger); and the Department of Pathology, St Barnabas Medical Center, Livingston, NJ (Dr Kim).

Footnotes
Accepted for publication August 5, 1990.

Read before the 14th Annual Surgical Symposium of the Association of Veterans Affairs Surgeons, Charleston, SC, May 20, 1990.

Reprint requests to the Department of Surgery, Loyola University Medical Center, 2160 S First Ave, Maywood, IL 60153 (Dr Greisler).

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