Metastatic potential of colon carcinoma. Expression of ABO/Lewis-related antigens
S. D. Hoff, T. Irimura, Y. Matsushita, D. M. Ota, K. R. Cleary and S. Hakomori
Department of General Surgery, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Four monoclonal antibodies specific to various Lewisx-related antigens were
tested for binding to monolayers and detergent extracts of the human colon
carcinoma cell line HT-29 P and its metastatic variant HT-29 LMM. Only
monoclonal antibody FH6 (antisialyl-dimeric Lex) bound more to the
metastatic variant compared with HT-29 P. Detergent extracts of human
tissues were then assayed for their FH6-binding activity. Normal colonic
mucosa displayed less FH6 binding than either Dukes stage B or D primary
colon carcinomas. Liver metastases from colorectal carcinoma demonstrated
greater binding than stage B and stage D primary tumors, and more than
normal liver. Expression of sialyl-dimeric Lex increases as the metastatic
potential of colorectal carcinomas increases, and is related to the
progression of colorectal cancer.