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Expression of ABO/Lewis-Related Antigens

Stuart D. Hoff, MD; Tatsuro Irimura, PhD; Yoshifumi Matsushita, MD; David M. Ota, MD; Karen R. Cleary, MD; Sen-itiroh Hakomori, MD

Arch Surg. 1990;125(2):206-209.

Abstract
• Four monoclonal antibodies specific to various Lewis^x-related antigens were tested for binding to monolayers and detergent extracts of the human colon carcinoma cell line HT-29 P and its metastatic variant HT-29 LMM. Only monoclonal antibody FH6 (antisialyl-dimeric Le^x) bound more to the metastatic variant compared with HT-29 P. Detergent extracts of human tissues were then assayed for their FH6-binding activity. Normal colonic mucosa displayed less FH6 binding than either Dukes stage B or D primary colon carcinomas. Liver metastases from colorectal carcinoma demonstrated greater binding than stage B and stage D primary tumors, and more than normal liver. Expression of sialyl-dimeric Le^x increases as the metastatic potential of colorectal carcinomas increases, and is related to the progression of colorectal cancer.

(Arch Surg. 1990;125:206-209)

Author Affiliations
From the Departments of General Surgery (Drs Hoff and Ota), Tumor Biology (Drs Irimura and Matsushita), and Pathology (Dr Cleary), University of Texas M. D. Anderson Cancer Center, Houston, and the Biomembrane Institute and University of Washington, Seattle (Dr Hakomori).

Footnotes
Accepted for publication November 15, 1989.

Read before the annual meeting of the Society of Surgical Oncology, San Francisco, Calif, May 22, 1989.

Reprints requests to the Department of General Surgery, University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030 (Dr Ota).

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