Changes associated with metastasis in B16-F1 melanoma cells surviving heat

S. D. Nathanson, R. F. Cerra, F. W. Hetzel, R. J. Zarbo, J. D. Crissman, R. Page, P. Anaya and P. Westrick
Department of Surgery, Henry Ford Hospital, Detroit, Mich. 48202.

Metastasis to distant sites is mediated by various receptors on the surface of tumor cells. B16-F1 melanomas surviving 43.5 degrees C heat in vitro for 15 minutes and cultured for 10 days bind significantly increased amounts of the basement membrane protein laminin. Motility of heat-resistant B16-F1 cells in vitro toward the chemoattractant laminin is significantly increased. The increased expression of putative laminin receptors may be associated with increased metastasis of melanomas after subcurative hyperthermia.