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Improved Results After Vaccine Modified With Recombinant Interferon Gamma

Robert K. Sigal, MD; Michael D. Lieberman, MD; John V. Reynolds, FRCS; Noel Williams, FRCS; Moritz M. Ziegler, MD; John M. Daly, MD

Arch Surg. 1990;125(3):308-312.

Abstract
•The purpose of this study was to determine if a 3-day in vitro culture of the murine neuroblastoma C1300 with 500 U/mL of recombinant interferon gamma resulted in a protective crossreactivity to parent C1300. Twenty A/J mice received either a vaccine of 1 x 10⁶ irradiated C1300 tumor cells intradermally or an equivalent innoculum of C1300 that had been incubated in recombinant interferon gamma (C1300*). One week later, all animals were rechallenged with 1 x 10⁶ viable C1300. Animals immunized with C1300* had a significantly delayed early tumor incidence that translated into a survival advantage for the group. At the time of tumor rechallenge, a significantly increased level of nonspecific systemic immunity was conferred by the C1300* immunization. Thus, modification of tumor with recombinant interferon gamma before introduction as a vaccine may improve that vaccine's protective capability.

(Arch Surg. 1990;125:308-312)

Author Affiliations
From the Harrison Department of Surgical Research, the University of Pennsylvania School of Medicine, Philadelphia (Drs Sigal, Lieberman, Reynolds, Williams, and Daly) and the Joseph Stokes Jr Research Institute, the Childrens Hospital of Philadelphia (Dr Ziegler). Dr Reynolds is now with the Hull (England) Royal Infirmary.

Footnotes
Accepted for publication December 4, 1989.

Read before the annual meeting of the Society of Surgical Oncology, May 22, 1989, San Francisco, Calif.

Reprint requests to the Division of Surgical Oncology, Hospital of the University of Pennsylvania, 3400 Spruce St, Philadelphia, PA 19104 (Dr Daly).

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