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An In Vitro Model for Colon Carcinogenesis

Susan E. Pories, MD; Ian C. Summerhayes, PhD; Glenn D. Steele, MD, PhD

Arch Surg. 1991;126(11):1387-1389.

Abstract
• Evaluation of molecular events in human colon polyps and tumors has revealed constitutive elevated expression of c-myc, activation of both ras and src proto-oncogenes, and allelic deletion events involving inactivation of putative regulatory genes, including p53.To evaluate the contribution of each of these events to colon carcinogenesis, it is desirable to establish epithelial cell lines representing different stages of neoplastic progression. Such in vitro models can be used to establish a primary role for different genes implicated in neoplastic transformation, identifying events involved in multistep carcinogenesis and delineating the factors modulating cellular transformation. We present herein a summary of such an in vitro model for colon carcinogenesis using the introduction of relevant genetic elements into normal mucosa to identify the molecular steps and accompanying cellular events underlying neoplastic progression in the colon.

(Arch Surg. 1991;126:1387-1389)

Author Affiliations
From the Department of Surgery, Laboratory of Cancer Biology, New England Deaconess Hospital, Boston, Mass.

Footnotes
Accepted for publication September 1, 1991.

Presented at the 44th Annual Cancer Symposium of the Society of Surgical Oncology, Orlando, Fla, March 26, 1991.

Reprint requests to New England Deaconess Hospital, Laboratory of Cancer Biology, 50 Binney St, Boston, MA 02115 (Dr Pories).

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