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Edward C. Lee, MD; Hee-Jong Woo, DVM, PhD; Cynthia A. Korzelius; Glenn D. Steele, Jr, MD, PhD; Arthur M. Mercurio, PhD

Arch Surg. 1991;126(12):1498-1502.

Abstract
• The interaction of tumor cells with laminin is thought to be critical in invasion and metastasis. We found that an endogenous lectin, carbohydrate-binding protein 35 (CBP-35), is the major laminin-binding protein on human colon carcinoma cells and that its surface expression suggests involvement in metastasis. We identified CBP-35 by lamininaffinity chromatography and immunoblotting. Surface expression of CBP-35 on eight human colon carcinoma cell lines was compared by flow cytometry. Poorly differentiated cell lines and DLD-2, a signet-ring carcinoma cell line, expressed more surface CBP-35 than well-differentiated cell lines. Poorly differentiated cell lines and DLD-2 are characterized as aggressive cell lines because they adhere to and invade through reconstituted basement membrane significantly better than well-differentiated cell lines. These data suggest that CBP-35 is involved in tumor cell-basement membrane interactions and that an increase in CBP-35 surface expression may facilitate metastatic potential of colon carcinoma cells.

(Arch Surg. 1991;126:1498-1502)

Author Affiliations
From the Laboratory of Cancer Biology, Deaconess Hospital, Harvard Medical School, Boston, Mass.

Footnotes
Accepted for publication September 1, 1991.

Presented at the 44th Annual Cancer Symposium of the Society of Surgical Oncology, Orlando, Fla, March 26, 1991.

Reprint requests to Laboratory of Cancer Biology, Deaconess Hospital, Harvard Medical School, 50 Binney St, Boston, MA 02115 (Dr Mercurio).

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