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Christopher C. Baker, MD; A. Tracy Niven-Fairchild; Alan Yamada, MD; Cheryl L. Caragnano; Thomas S. Kupper, MD

Arch Surg. 1991;126(2):253-258.

Abstract
• Following cecal ligation and puncture with a 25-gauge needle, endotoxin-sensitive C₃H/HeN mice have a 45% mortality compared with no mortality in endotoxin-resistant C₃H/HeJ mice. Macrophage production of interleukin 1 and antigen presentation were studied in these two strains of mice following cecal ligation and puncture at 2, 4, 8, 16, and 24 hours and at 2, 4, 6, and 8 days. Splenic macrophages were cultured with a T-helper cell clone (D10.G4.1), and antigen presentation and interleukin 1 production were measured by D10.G4.1 proliferation. Macrophage antigen presentation by C₃H/HeJ mice was markedly increased compared with that in C₃H/HeN mice at all times after cecal ligation and puncture, most strikingly at 2 days (185 740 cpm for C₃H/HeJ mice vs 30 300 for C₃H/HeN mice). Macrophage interleukin 1 production was significantly increased in C₃H/HeJ mice vs C₃H/HeN mice at all times after cecal ligation and puncture (except at 2 days) and was maximal at 8 days (25 000 cpm for C3H/HeJ mice vs 5190 for C₃H/HeN mice). These data suggest that the differences in mortality after cecal ligation and puncture between these two strains of mice may relate to a supranormal response of macrophages of C₃H/HeJ mice or to an inadequate response of macrophages of C₃H/HeN mice.

(Arch Surg. 1991;126:253-258)

Author Affiliations
From the Departments of Surgery, University of North Carolina School of Medicine, Chapel Hill (Dr Baker), Yale University School of Medicine, New Haven, Conn (Mss Niven-Fairchild and Caragnano), and UCLA School of Medicine (Dr Yamada); and the Department of Dermatology, Washington University School of Medicine, St Louis, Mo (Dr Kupper).

Footnotes
Accepted for publication October 28, 1990.

Read before the Tenth Anniversary Meeting of the Surgical Infection Society, Cincinnati, Ohio, June 16, 1990.

Reprints not available.

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