This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

Dilipkumar Parekh, MD; Jin Ishizuka, MD; Courtney M. Townsend, Jr, MD; Bernard E. Haber, PhD; R. Daniel Beauchamp, MD; Srinivasan Rajaraman, MD; George Karp; Jell Hsieh, MS; James C. Thompson, MD

Arch Surg. 1991;126(4):467-472.

Abstract
• Advanced gastrointestinal endocrine tumors respond poorly to conventional chemotherapy. In this study we examined the effects of two agents that promote cellular differentiation, sodium butyrate and hexamethylene bisacetamide, on the in vitro growth and secretory responses of a human pancreatic carcinoid (BON) and human gastrinoma (PT-2 and PT-SM) cell lines that have been established in our laboratory. We found that both sodium butyrate and hexamethylene bisacetamide strongly inhibited growth of BON, PT-2, and PT-SM cells. With continuous exposure of BON cells to sodium butyrate (2 mmol/L), the doubling time was prolonged, from 60 hours in controls to 156 hours, and saturation density was reduced to 28% that of controls. Hexamethylene bisacetamide (4 mmol/L) reduced saturation density to 37% that of controls in BON cells and prolonged the doubling time, from 60 hours to 103 hours. Antiproliferative effects of similar magnitudes were observed in the gastrinoma cell lines. In contrast, differential effects were produced on amine biosynthesis in BON cells; sodium butyrate stimulated levels of 5-hydroxytryptamine in the cells, whereas hexamethylene bisacetamide caused a profound dose-dependent inhibition of amine biosynthesis. The sigificant antiproliferative activity of sodium butyrate and hexamethylene bisacetamide and the inhibitory effects of hexamethylene bisacetamide on amine biosynthesis warrant evaluation of these agents or analogues for treatment of metastatic carcinoid and gastrinoma.

(Arch Surg. 1991;126:467-472)

Author Affiliations
From the Departments of Surgery (Drs Parekh, Ishizuka, Townsend, Beauchamp, and Thompson and Ms Hsieh) and Pathology (Dr Rajaraman) and the Marine Biology Institute (Dr Haber), University of Texas Medical Branch, Galveston.

Footnotes
Accepted for publication January 12, 1990.

Read before the 43rd Annual Cancer Symposium of the Society of Surgical Oncology, Washington, DC, May 21, 1990.

Reprint requests to Department of Surgery, University of Texas Medical Branch, Galveston, TX 77550 (Dr Parekh).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati     What's this?