This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

Agustin A. Rodriguez, MD; Wayne W. LaMorte, MD, PhD; Lawrence M. Hanrahan, MD; Susan R. Hopkins; J. Conor O'Keane, MD; Riad Cachecho, MD; Erwin F. Hirsch, MD

Arch Surg. 1991;126(6):767-772.

Abstract
• Lack of a reproducible model to quantitatively assess hepatocellular injury following ischemia has made it difficult to assess new strategies for minimizing hepatic injury. We studied the progression of hepatocellular injury after ischemia and ischemia with reperfusion in rats. Irreversible injury was quantitated using a triphenyltetrazolium chloride assay that was shown to correlate with ultrastructural changes. Adenosine triphosphate decreased to 36% of basal values after 30 minutes, but returned to normal with reperfusion with no decrease in viability. In contrast, viability fell by 30% after 60 minutes of ischemia, and by 64% when 60 minutes of ischemia was followed by reperfusion. We conclude that reperfusion of ischemic liver increases the degree of irreversible damage. The model employed here seems to be useful for studying ischemic and reperfusion injury in the liver.

(Arch Surg. 1991;126:767-772)

Author Affiliations
From the Department of Surgery, Sears Surgical Laboratory (Drs Rodriguez, LaMorte, Hanrahan, Cachecho, and Hirsch and Ms Hopkins), and Mallory Institute of Pathology (Dr O'Keane), Boston (Mass) University School of Medicine.

Footnotes
Accepted for publication February 10, 1991.

Reprint requests to Surgical Research Section, Room L-908, Boston University School of Medicine, 80 E Concord St, Boston, MA 02118 (Dr LaMorte).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Liver Transplantation in Rats Using Small-for-Size Grafts: A Study of Hemodynamic and Morphological Changes
Man et al.
Arch Surg 2001;136:280-285.
ABSTRACT | FULL TEXT  

Optimal Cycles of Hepatic Ischemia and Reperfusion for Intermittent Pedicle Clamping During Liver Surgery
Horiuchi et al.
Arch Surg 1995;130:754-758.
ABSTRACT