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Renato S. Poggetti, MD; Frederick A. Moore, MD; Ernest E. Moore, MD; Denis D. Bensard, MD; Benjamin O. Anderson, MD; Anirban Banejee, PhD

Arch Surg. 1992;127(2):175-179.

Abstract
• The purposes of this study were to characterize the temporal relationship of distant organ dysfunction after mesenteric ischemia/reperfusion (l/R), and to ascertain if the neutrophil is critical to this process. Normal and neutrophildepleted rats (vinblastine sulfate, 0.75 mg/kg intravenously) underwent 45 minutes of superior mesenteric artery occlusion and after 0, 6, and 18 hours of reperfusion, blood was sampled and liver and lungs were harvested. Iodine 125 albumin leak was used as a marker for pulmonary and liver injury, and serum acetoacetate/3-hydroxybutyrate (AcAc/3-OHB) was used as an index of hepatic mitochondrial redox state. Gut l/R at 6 hours increased ¹²⁵l-albumin lung/blood ratio (gut l/R, 0.077±0.006; control, 0.045±0.004) and ¹²⁵l-albumin liver/blood ratio (gut l/R, 0.120±0.007; control, 0.077±0.003), while AcAc/3-OHB decreased significantly (gut l/R, 0.420±0.040; control, 0.880±0.120). Neutrophil depletion eliminated these changes at 6 hours (blood AcAc/3-OHB, 0.720±0.100; ¹²⁵l liver/blood, 0.068±0.006; ¹²⁵ lung/blood, 0.046±0.007). We conclude the following: (1) intestinal l/R produces simultaneous liver and lung injury; (2) injury was present at 6 hours but is reversed at 18 hours; and (3) the l/R-induced liver and lung injuries were neutrophil mediated.

(Arch Surg. 1992;127:175-179)

Author Affiliations
From the Departments of Surgery, Denver (Colo) General Hospital (Drs Poggetti, F. Moore, and E. Moore) and the University of Colorado Health Sciences Center (Drs F. Moore, E. Moore, Bensard, Anderson, and Banerjee), Denver. Dr Poggetti is now with the Department of Trauma, University of Saã Paulo, Brazil.

Footnotes
Accepted for publication November 2, 1991.

Presented at the 11th Annual Meeting of the Surgical Infection Society, Fort Lauderdale, Fla, April 9, 1991.

Reprint requests to Department of Surgery, Box C-311, University of Colorado Health Sciences Center, 4200 E Ninth Ave, Denver, CO 80262 (Dr Banerjee).

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