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Alfred Ayala, PhD; Jennifer B. Knotts, MD; Wolfgang Ertel, MD; Michelle M. Perrin; Mary H. Morrison, MS; Irshad H. Chaudry, PhD

Arch Surg. 1993;128(1):89-95.

Abstract
• We examined whether (1) there is an association between elevated circulating levels of transforming growth factor–β (TGF-β) and splenocyte dysfunction during sepsis, and (2) administration of monoclonal antibodies to interleukin 6 (an inducer of TGF-β release) or TGF-β could ablate these changes. Blood and splenocytes were obtained from C3H/HeN mice at 1, 4, or 24 hours following cecal ligation and puncture or sham operation. Only at 24 hours after cecal ligation and puncture was there an association between elevated blood TGF-β value and depressed splenocyte interleukin 2 release. Administration of monoclonal antibodies against interleukin 6, but not against TGF-β (intraperitoneally immediately following cecal ligation and puncture), significantly decreased the blood levels of TGF-β at 24 hours following cecal ligation and puncture and improved splenocyte interleukin 2 release. Thus, the judicious use of monoclonal antibodies against interleukin 6 may block the subsequent elevation of TGF-β, thereby attenuating host immunosuppression during sepsis.

(Arch Surg. 1993;128:89-95)

Author Affiliations
From the Shock and Trauma Research Laboratories, Departments of Surgery (Drs Ayala, Knotts, Ertel, and Chaudry, and Mss Perrin and Morrison), Microbiology (Dr Ayala), and Physiology (Dr Chaudry), Michigan State University, East Lansing.

Footnotes
Accepted for publication August 15, 1992.

Presented at the 12th Annual Meeting of the Surgical Infection Society, Los Angeles, Calif, April 11, 1992.

Reprint requests to Department of Surgery, Michigan State University, B424 Clinical Center, East Lansing, MI 48824 (Dr Chaudry).

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