The activation of bone marrow macrophages 24 hours after thermal injury
C. K. Ogle, X. Guo, J. W. Alexander, R. Fukushima and J. D. Ogle
Shriners Burns Institute, Cincinnati, Ohio 45229-3095.
We determined the effect of thermal injury on the in vitro production of
the immunoactive substances tumor necrosis factor, interleukin 1,
prostaglandin E2, and complement component C3 by
lipopolysaccharide-stimulated guinea pig bone marrow macrophages and on the
cytotoxicity of these cells. Macrophages from burned animals produced
different amounts of these mediators compared with unburned animals at
certain culture times, suggesting that thermal injury could program the
bone marrow cells to respond differently from normal cells to in vitro
stimulation with lipopolysaccharide. Also, the macrophages from burned
animals displayed greater cytotoxicity towards L929 target cells. These
results suggest that there is a complex interaction among cellular
secretory products, especially after thermal injury, that may be important
in host defense.
