Determination of tumor aggressiveness in colorectal cancer by K-ras-2 analysis

S. D. Finkelstein, R. Sayegh, A. Bakker and P. Swalsky
Department of Pathology, Rhode Island Hospital, Brown University, Providence 02903.

Markers that predict tumor aggressiveness on a case-by-case basis would enable individualization and optimization of oncologic therapy. To achieve this goal, the presence and specific type of K-ras-2 point mutation was determined from formalin-fixed, paraffin-embedded tissue sites in 247 primary and 166 metastatic-recurrent colorectal adenocarcinomas, using a novel approach consisting of topographic tissue selection, DNA amplification, and direct sequencing applicable to large and needle-biopsy-sized specimens. The results provide the basis for a genotypic classification of colorectal cancer capable of predicting individual tumor aggressiveness, including the pattern and extent of metastasis.

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

K-ras Codon-Specific Mutations Produce Distinctive Metabolic Phenotypes in Human Fibroblasts
Vizan et al.
Cancer Res. 2005;65:5512-5515.
ABSTRACT | FULL TEXT  

Inverse Relationship between Microsatellite Instability and K-ras and p53 Gene Alterations in Colon Cancer
Samowitz et al.
Am. J. Pathol. 2001;158:1517-1524.
ABSTRACT | FULL TEXT  

K-ras Codon 12 Mutation Induces Higher Level of Resistance to Apoptosis and Predisposition to Anchorage-independent Growth Than Codon 13 Mutation or Proto-Oncogene Overexpression
Guerrero et al.
Cancer Res. 2000;60:6750-6756.
ABSTRACT | FULL TEXT  

Relationship of Ki-ras Mutations in Colon Cancers to Tumor Location, Stage, and Survival: A Population-based Study
Samowitz et al.
Cancer Epidemiol. Biomarkers Prev. 2000;9:1193-1197.
ABSTRACT | FULL TEXT  

Mutation Detection in K-ras, BRCA1, BRCA2, and p53 Using PCR/LDR and a Universal DNA Microarray
FAVIS and BARANY
Ann. N. Y. Acad. Sci. 2000;906:39-43.
ABSTRACT | FULL TEXT  

Prognostic Significance of K-ras Codon 12 Mutations in Patients With Resected Stage I and II Non–Small-Cell Lung Cancer
Graziano et al.
JCO 1999;17:668-668.
ABSTRACT | FULL TEXT  

Oncogenic Ki-ras but Not Oncogenic Ha-ras Blocks Integrin beta 1-Chain Maturation in Colon Epithelial Cells
Yan et al.
J. Biol. Chem. 1997;272:30928-30936.
ABSTRACT | FULL TEXT  

Oncogenic c-Ki-ras but Not Oncogenic c-Ha-ras Up-regulates CEA Expression and Disrupts Basolateral Polarity in Colon Epithelial Cells
Yan et al.
J. Biol. Chem. 1997;272:27902-27907.
ABSTRACT | FULL TEXT  

Allelic Loss of Chromosome 18q and Prognosis in Colorectal Cancer
Jen et al.
NEJM 1994;331:213-221.
ABSTRACT | FULL TEXT  

Differential Activation of the Rac Pathway by Ha-Ras and K-Ras
Walsh and Bar-Sagi
J. Biol. Chem. 2001;276:15609-15615.
ABSTRACT | FULL TEXT