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Andrew Saxe, MD; Glenn Gibson

Arch Surg. 1993;128(8):865-869.

Abstract
Objective
To measure lithium chloride—stimulated incorporation of tritiated thymidine (³H-TdR) and bromodeoxyuridine (BrdU) by human parathyroid cells as an indicator of lithium's growth-promoting properties.

Design
Cell suspensions derived from abnormal human parathyroid tissue were incubated for 5 days with graded concentrations of lithium and pulsed with either 0.074 MBq of 3H-TdR or 0.6 µg of BrdU. The ³H-TdR incorporation was measured by counting cell filtrates in a beta counter, and nuclear BrdU incorporation was determined by counting nuclei positively stained by an antibody to BrdU. Counts of BrdU-positive cells by two observers unaware of incubation conditions were correlated with ³H-TdR counts.

Results
Cell suspensions derived from adenomatous and hyperplastic parathyroid glands demonstrated a dose-related increase in ³H-TdR incorporation. There was close correlation between the two observers' counts of BrdU-positive cells (r=0.70, P=.01) and between each observer's counts and ³H-TdR counts (r=0.70, P=.02, and r=0.90, P=.01, respectively).

Conclusions
Lithium stimulates in vitro incorporation of ³H-TdR and BrdU by abnormal human parathyroid cells, consistent with a growth-promoting effect.

(Arch Surg. 1993;128:865-869)

Author Affiliations
From the Department of Surgery and the DeRoy Surgical Research Laboratory, Sinai Hospital, Detroit, Mich.

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