You are seeing this message because your Web browser does not support basic Web standards. Find out more about why this message is appearing and what you can do to make your experience on this site better.


Advertisement

ABOUT ARCHIVES
Advanced Search

Welcome   | My Account | E-mail Alerts | RSS | Access Rights | Sign In


  Vol. 129 No. 12, December 1994 TABLE OF CONTENTS
  Online Only
 •  Online First Table of
Contents
  Papers
 •Online Features
 This Article
 •References
 •Full text PDF
 • Reply to article
 •Send to a friend
 • Save in My Folder
 •Save to citation manager
 •Permissions
 Citing Articles
 •Citation map
 •Citing articles on HighWire
 •Citing articles on Web of Science (17)
 •Contact me when this article is cited
 Related Content
 •Similar articles in this journal
 Social Bookmarking
  Add to CiteULike Add to Connotea Add to Delicious Add to Digg Add to Facebook Add to Reddit Add to Technorati Add to Twitter What's this?

The Local Role of Tumor Necrosis Factor {alpha} in the Modulation of Neutrophil Function at Sites of Inflammation

John Yee, MD; Nicolas V. Christou, MD, PhD, FRCSC

Arch Surg. 1994;129(12):1249-1255.


Abstract



Objective
To examine the hypothesis that tumor necrosis factor {alpha} (TNF-{alpha}) is an important local modulator of neutrophil function in the inflammatory microenvironment.

Design
In vitro studies of host defense.

Patients
A volunteer sample of healthy subjects.

Intervention
Exudative neutrophils were collected from skin-blister chambers and functionally compared with blood neutrophils.

Methods
Tumor necrosis factor {alpha} levels at sites of inflammation and neutrophil exudation were determined and compared with serum concentrations. Flow cytometry was used to evaluate neutrophil microbicidal activity and N-formyl-methionyl-leucyl-phenylalanine—induced changes in intracellular calcium and superoxide production. In vitro TNF-{alpha} was used to evaluate the nature and dose response of TNF-{alpha}–induced changes in neutrophil function.

Results
Exudative neutrophils have an increased responsiveness to subsequent N-formyl-methionyl-leucyl-phenylalanine stimulation, as determined by changes in intracellular calcium. Microbicidal activity and superoxide production are also up-regulated compared with circulating neutrophils. The exudative microenvironment contains TNF-{alpha} at local levels that are capable of significantly enhancing neutrophil host defense.

Conclusions
Tumor necrosis factor {alpha} may serve to enhance neutrophil function at sites of inflammation. Neutrophils become more cytotoxic and have an enhanced ability to respond to weak environmental signals.

(Arch Surg. 1994;129:1249-1255)



Author Affiliations



From the Departments of Surgery (Drs Yee and Christou) and Microbiology (Dr Christou), Royal Victoria Hospital, McGill University, Montréal, Quebec.



Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Delicious Delicious   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Hostile Marital Interactions, Proinflammatory Cytokine Production, and Wound Healing
Kiecolt-Glaser et al.
Arch Gen Psychiatry 2005;62:1377-1384.
ABSTRACT | FULL TEXT  

Alanyl-Glutamine Enriched Total Parenteral Nutrition Improves Local, Systemic, and Remote Organ Responses to Intraperitoneal Bacterial Challenge
Lin et al.
JPEN J Parenter Enteral Nutr 2001;25:346-351.
ABSTRACT  

More than destructive: neutrophil-derived serine proteases in cytokine bioactivity control
Bank and Ansorge
J. Leukoc. Biol. 2001;69:197-206.
ABSTRACT | FULL TEXT  





HOME | CURRENT ISSUE | PAST ISSUES | TOPIC COLLECTIONS | CME | PHYSICIAN JOBS | SUBMIT | SUBSCRIBE | HELP
CONDITIONS OF USE | PRIVACY POLICY | CONTACT US | SITE MAP
 
© 1994 American Medical Association. All Rights Reserved.