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  Vol. 129 No. 12, December 1994 TABLE OF CONTENTS
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Nitric Oxide Inhibition Normalizes Splenocyte Interleukin-10 Synthesis in Murine Thermal Injury

Lena M. Napolitano, MD; Cara Campbell

Arch Surg. 1994;129(12):1276-1283.


Abstract

Objective
To examine the effect of nitric oxide inhibition on cytokine production and immunologic function in a murine thermal-injury and an alcohol (ETOH)–ingestion model.

Design
Randomized controlled experiment.

Setting
University surgical research laboratory.

Animals
Forty male Balb/C mice.

Interventions
Animals were randomized to four groups: normal saline solution—sham (NS-sham), ETOH-sham, NS-burn, and ETOH-burn. Animals received 20% ETOH or NS daily for 14 days by gavage. A 20% full-thickness burn was induced 4 hours after the last dose of ETOH or NS was administered. Animals were killed 4 days after the burn was induced.

Main Outcome Measures
Splenocytes were harvested and stimulated with the mitogens lipopolysaccharide or concanavalin A. These mitogen-stimulated splenoctye cultures had the addition of exogenous N-monomethyl-L-arginine (2.5 or 10 µg/mL), a nitric oxide synthase inhibitor. Splenocyte production of interleukin-10 (IL-10), interferon-{gamma}, nitrite, and prostaglandin E2 were measured, and lymphocyte proliferative response was examined.

Results
Interleukin-10 and interferon-{gamma} production were significantly suppressed in thermal injury, and lymphocyte proliferative response was markedly reduced. Exogenous N-monomethyl-L-arginine normalized splenocyte IL-10 production in a dose-dependent manner in NS-burn and ETOH-burn groups, improved lymphocyte proliferative response, and significantly decreased splenocyte nitrite production. Interferon-{gamma} release was not up-regulated by N-monomethyl-L-arginine.

Conclusions
Thermal injury is associated with a suppression of splenocyte IL-10 production and lymphocyte proliferative response. Inhibition of nitric oxide synthesis normalized IL-10 production and significantly improved splenocyte proliferative response. These data suggest that nitric oxide is an important modulator of cytokine regulation and immunologic function in thermal injury, thereby ultimately influencing host defense.

(Arch Surg. 1994;129:1276-1283)



Author Affiliations

From the Department of Surgery, the University of Massachusetts, Worcester.



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