You are seeing this message because your Web browser does not support basic Web standards. Find out more about why this message is appearing and what you can do to make your experience on this site better.


ABOUT ARCHIVES
Advanced Search

Welcome   | My Account | E-mail Alerts | Access Rights | Sign In


  Vol. 131 No. 12, December 1996 TABLE OF CONTENTS
  Archives
  •  Online Features
  PAPERS
 This Article
 •References
 •Full text PDF
 •Send to a friend
 • Save in My Folder
 •Save to citation manager
 •Permissions
 Citing Articles
 •Citation map
 •Citing articles on HighWire
 •Contact me when this article is cited
 Related Content
 •Similar articles in this journal
 Social Bookmarking
  Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit Add to Technorati
What's this?

Glucagon Inhibits Hepatocyte Nitric Oxide Synthesis

Brian G. Harbrecht, MD; Elizabeth M. Wirant; Young-Myeong Kim, PhD; Timothy R. Billiar, MD

Arch Surg. 1996;131(12):1266-1272.


Abstract

Objective
To investigate the effects of glucagon on nitric oxide (NO) synthesis in cultured rat hepatocytes.

Setting
Laboratory.

Materials
Male Sprague-Dawley rats (weight, 200-250 g).

Interventions
Isolated rat hepatocytes were cultured with interleukin-1 to stimulate NO synthesis. Glucagon was added at increasing concentrations (from 10–9 to 2x 10–5 mol/L) at the time of interleukin-1 stimulation. Selected cultures were treated with the adenylate cyclase inhibitor, SQ 22 536 (from 10–5 to 10–3 mol/L).

Main Outcome Measures
Nitric oxide synthesis was assessed by measuring the concentrations of culture supernatant nitrite and nitrite plus nitrate, hepatocyte nitric oxide synthase-2 (NOS-2) messenger RNA (mRNA), and NOS-2 protein.

Results
Interleukin-1 stimulated hepatocyte NO synthesis, and this synthesis was inhibited by glucagon in a dose-dependent manner. Glucagon inhibited the accumulation of supernatant nitrite and the expression of NOS-2 mRNA and NOS-2 protein. SQ 22 536 restored glucagon-induced decreases in NO synthesis.

Conclusions
Glucagon inhibits NO synthesis in interleukin-1–stimulated hepatocytes in vitro. This inhibition seems to be mediated by glucagon-induced changes in cyclic adenosine monophosphate.

Arch Surg. 1996;131:1266-1272



Author Affiliations

From the Department of Surgery, University of Pittsburgh, Pittsburgh, Pa.



Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Effects of simulated hyperglycemia, insulin, and glucagon on endothelial nitric oxide synthase expression
Ding et al.
Am. J. Physiol. Endocrinol. Metab. 2000;279:E11-E17.
ABSTRACT | FULL TEXT  

Regulation of the expression of the inflammatory nitric oxide synthase (NOS2) by cyclic AMP
GALEA and FEINSTEIN
FASEB J. 1999;13:2125-2137.
ABSTRACT | FULL TEXT  

Differential regulation of arginases and inducible nitric oxide synthase in murine macrophage cells
Morris et al.
Am. J. Physiol. Endocrinol. Metab. 1998;275:E740-E747.
ABSTRACT | FULL TEXT  





HOME | CURRENT ISSUE | PAST ISSUES | TOPIC COLLECTIONS | CME | SUBMIT | SUBSCRIBE | HELP
CONDITIONS OF USE | PRIVACY POLICY | CONTACT US | SITE MAP
 
© 1996 American Medical Association. All Rights Reserved.