Complement C3 Production in Human Intestinal Epithelial Cells Is Regulated by Interleukin 1β and Tumor Necrosis Factor 
Ryan Moon, MD;
Alexander A. Parikh, MD;
Csaba Szabo, MD, PhD;
Josef E. Fischer, MD;
Andrew L. Salzman, MD;
Per-Olof Hasseigren, MD
Arch Surg. 1997;132(12):1289-1293.
Abstract
| |
Background
Sepsis and endotoxemia are associated with increased mucosal production of complement component C3; the enterocyte may be a source of C3 in these conditions.
Objective
To test the hypothesis that interleukin 1β (IL-1β) and tumor necrosis factor  (TNF-) regulate the production of C3 in the enterocyte at the transcriptional level and that this regulation is potentiated by interferon gamma (IFN- ).
Methods
Cultured Caco-2 cells, a human intestinal epithelial cell line, were treated with various concentrations of human recombinant IL-1 β (0.005-1.25 ng/mL) or TNF- (1-1000 U/mL) with or without the addition of IFN- (250 U/mL). C3 levels in the culture medium were measured by enzyme-linked immunosorbent assay and cellular messenger RNA levels by Northern blot analysis.
Results
Treatment of the Caco-2 cells with IL-1β or TNF- resulted in a time- and dose-dependent increase in C3 production. The use of IFN- alone did not affect C3 production but potentiated the effect of IL-1β and TNF- in a synergistic manner. C3 messenger RNA levels were increased following stimulation with either cytokine.
Conclusions
C3 production in the enterocyte is regulated by IL-1β and TNF- at the transcriptional level, and this response is potentiated by TNF-. The results suggest that C3 production in the intestinal mucosa may be regulated locally by cytokines in a paracrine or autocrine manner.
Arch Surg. 1997;132:1289-1293
Author Affiliations
From the Department of Surgery, University of Cincinnati Medical Center (Drs Moon, Fischer, and Hasselgren); the Shriners Burn Institute (Dr Parikh); the Division of Critical Care, Children's Hospital Medical Center (Drs Szabo and Salzman), Cincinnati, Ohio.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter
What's this?
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
Proteasome inhibitors induce heat shock response and increase IL-6 expression in human intestinal epithelial cells
Pritts et al.
Am. J. Physiol. Regul. Integr. Comp. Physiol. 2002;282:R1016-R1026.
ABSTRACT
| FULL TEXT
Endotoxemia in mice stimulates production of complement C3 and serum amyloid A in mucosa of small intestine
Wang et al.
Am. J. Physiol. Regul. Integr. Comp. Physiol. 1998;275:R1584-R1592.
ABSTRACT
| FULL TEXT
|
