
Posttraumatic Auto-oxidative Polymorphonuclear Neutrophil Receptor Injury Predicts the Development of Nosocomial Infection
H. Hank Simms, MD;
Ronald D'Amico
Arch Surg. 1997;132(2):171-177.
Abstract
 |  |
Objective To determine the mechanisms and prevalence of posttraumatic auto-oxidative receptor injury and immune suppression to subsequent nosocomial infections.
Design Purified polymorphonuclear neutrophils from 30 critically ill trauma patients (mean [±SD] Injury Severity Scores, 21.5±7.3) were incubated with glucose and glucose-oxidase to generate superoxide anion. Subcellular fractionations were performed with iodine I 125 monoclonal antibodies against the Fc receptors CD32w and CD16 and complement receptors CD35 and CD11b/CD18. Plasma membrane expression of these receptors was then determined during the first week of hospitalization.
Setting Surgical intensive care unit in a university-affiliated hospital.
Results Twenty-three (77%) of 30 patients had persistent auto-oxidative reduction in Fc and complement receptors induced by glucose and glucose-oxidase. Nosocomial infections occurred in 20 (87%) of 23 patients with auto-oxidative injury vs 1 (14%) of 7 patients without auto-oxidative receptor injury (P<.01, unpaired t test). Patients without auto-oxidative injury had expression for each receptor no different from buffer control.
Conclusions Critically ill trauma patients have auto-oxidative receptor injury, which is closely linked with the development of nosocomial infections. These results provide a biological basis for the early use of auto-oxidants in critically ill trauma patients.
Arch Surg. 1997;132:171-177
Author Affiliations
From the Department of Surgery, Rhode Island Hospital/Brown University School of Medicine, Providence.
CiteULike Connotea Del.icio.us Digg Reddit Technorati Twitter
What's this?
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
 |
Bacterial Clearance and Cytokine Profiles in a Murine Model of Postsurgical Nosocomial Pneumonia
Manderscheid et al.
CVI 2004;11:742-751.
ABSTRACT
| FULL TEXT
Lymphocyte DNA damage precedes DNA repair or cell death after orthopaedic surgery under general anaesthesia
Alleva et al.
Mutagenesis 2003;18:423-428.
ABSTRACT
| FULL TEXT
Mitochondrial Perturbations and Oxidant Stress in Lymphocytes From Patients Undergoing Surgery and General Anesthesia
Delogu et al.
Arch Surg 2001;136:1190-1196.
ABSTRACT
| FULL TEXT
The In Vivo Effects of General and Epidural Anesthesia on Human Immune Function
Procopio et al.
Anesth. Analg. 2001;93:460-465.
ABSTRACT
| FULL TEXT
Differences Between Bacterial Species Shown by Simultaneous Assessment of Neutrophil Phagocytosis and Generation of Reactive Oxygen Intermediates in Trauma Patients
Taylor et al.
Arch Surg 1999;134:1222-1228.
ABSTRACT
| FULL TEXT
|