This Article  • Full text  • PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Citing articles on ISI (11)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Topic Collections  • Surgery, Other  • Alert me on articles by topic

Daniel D. Lozano, MD; Edward A. Kahl; Howard P. Wong, MD; Linda L. Stephenson; William A. Zamboni, MD

Arch Surg. 1999;134:1079-1081.

Hypothesis  Treatment with anti–L-selectin monoclonal antibody will reduce venular neutrophil-endothelial rolling (flux and velocity) and adhesion associated with ischemia reperfusion injury in rat skeletal muscle.

Design  Prospective, randomized experimental trials.

Setting  Basic science research laboratory.

Materials  Male Wistar rats weighing 109±5 g (mean±SEM).

Interventions  Gracilis pedicle muscle flaps were elevated and microcirculation was observed by intravital microscopy. Two groups were evaluated: (1) the control group, which received 4 hours of global ischemia, and (2) the experimental group, which received 4 hours of global ischemia, plus treatment with anti–L-selectin monoclonal antibody 30 minutes before reperfusion.

Main Outcome Measures  The number of rolling and adherent leukocytes in postcapillary venules were counted in the 2 groups at baseline and at 1 through 5, 10, 15, 20, 30, 45, and 60 minutes of reperfusion.

Results  Treatment with the monoclonal antibody to L-selectin significantly reduced the number of rolling leukocytes (flux) at 2 through 5, 20, 30, 45, and 60 minutes of reperfusion compared with controls (P<.05). Use of the monoclonal antibody significantly reduced the number of adherent neutrophils at 5, 10, 15, 20, 30, 45, and 60 minutes of reperfusion (P<.05). There was no significant difference in leukocyte velocity.

Conclusion  L-Selectin plays a significant role in leukocyte rolling and adherence to venular endothelium in rat skeletal muscle ischemia reperfusion injury.

From the Division of Plastic Surgery, Microsurgery and Hyperbaric Laboratory, School of Medicine (Drs Lozano, Wong, and Zamboni and Ms Stephenson), and Department of Biology (Mr Kahl), University of Nevada, Las Vegas.

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Hematopoietic Stem Cell Mobilization Therapy Accelerates Recovery of Renal Function Independent of Stem Cell Contribution
Stokman et al.
J. Am. Soc. Nephrol. 2005;16:1684-1692.
ABSTRACT | FULL TEXT  

P-selectin inhibition suppresses muscle regeneration following injury
Baker et al.
J. Leukoc. Biol. 2004;76:352-358.
ABSTRACT | FULL TEXT  

Suppressive effects of selectin inhibitor SKK-60060 on the leucocyte infiltration during endotoxin induced uveitis
Yamashiro et al.
Br. J. Ophthalmol. 2003;87:476-480.
ABSTRACT | FULL TEXT  

Hypotonicity induces L-selectin shedding in human neutrophils
Kaba and Knauf
Am. J. Physiol. Cell Physiol. 2001;281:C1403-C1407.
ABSTRACT | FULL TEXT