This Article  • Full text  • PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Citing articles on ISI (8)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Topic Collections  • Burns  • Surgical Cytokines  • Nutritional and Metabolic Disorders  • Alert me on articles by topic

Marc G. Jeschke, MD; Robert E. Barrow, PhD; J. Regino Perez-Polo, PhD; David N. Herndon, MD

Arch Surg. 1999;134:1098-1102.

Hypothesis  Cholesterol-containing cationic liposomes alone modulate the acute-phase response and cytokine expression in thermally injured rats and are an effective delivery system for gene therapy in trauma.

Setting  Laboratory.

Intervention  Fifty-six adult male Sprague-Dawley rats with a full-thickness scald burn covering 60% of total body surface area were randomly divided into 2 groups to receive either intravenous injections of cholesterol-containing cationic liposomes or saline (control).

Main Outcome Measures  Body weights, muscle and liver dry-wet weights, serum levels of constitutive hepatic proteins, acute-phase protein levels, and cytokine levels were determined at 1, 2, 5, and 7 days after thermal injury.

Results  Rats receiving cholesterol-containing cationic liposomes had less body weight loss, increased serum transferrin levels, and decreased serum ₁-acid glycoprotein levels when compared with controls (P<.05). Serum interleukin 1 and tumor necrosis factor levels were decreased in rats receiving liposomes at 1 and 2 days after burn compared with controls (P<.05).

Conclusions  These results suggest that cholesterol-containing cationic liposomes alone may have a beneficial effect in modulating the hypermetabolic response after burn injury by decreasing type 1 acute-phase proteins and the expression of the proinflammatory cytokines interleukin 1 and tumor necrosis factor . Therefore, cholesterol-containing cationic liposomes appear to be suitable as a delivery system for gene therapy in trauma.

From the Departments of Surgery (Drs Jeschke, Barrow, and Herndon) and Human Biological Chemistry and Genetics (Dr Perez-Polo), Shriners Burns Hospital, Galveston, Tex.

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

IGF-I gene transfer effects on inflammatory elements present after thermal trauma
Dasu et al.
Am. J. Physiol. Regul. Integr. Comp. Physiol. 2003;285:R741-R746.
ABSTRACT | FULL TEXT  

Effect of Multiple Gene Transfers of Insulinlike Growth Factor I Complementary DNA Gene Constructs in Rats After Thermal Injury
Jeschke et al.
Arch Surg 1999;134:1137-1141.
ABSTRACT | FULL TEXT