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Transforming Growth Factor ₃ Promotes Fascial Wound Healing in a New Animal Model

John W. Tyrone, MD; Jeffrey R. Marcus, MD; Steven R. Bonomo, MD; Jon E. Mogford, PhD; Yuping Xia, PhD; Thomas A. Mustoe, MD

Arch Surg. 2000;135:1154-1159.

Hypothesis  Transforming growth factor ₃ (TGF-₃) promotes fascial wound healing in a new animal model, as measured by wound breaking strength, collagen deposition, and cellular proliferation.

Design/Intervention  Bilateral, longitudinal incisions were made in the anterior rectus sheaths of 24 male New Zealand white rabbits. One incision was treated with 1 µg of TGF-₃; the contralateral incision served as a control. The wounds were harvested at 1, 2, 3, 4, 6, and 8 weeks after creation ("wounding").

Main Outcome Measures  Wound tissue was tested for breaking strength using a tensiometer and processed for histological examination of collagen deposition and cellular proliferation at all time points after wounding. Collagen deposition and cellular proliferation were measured in histological cross sections of wounds with Masson trichrome staining and proliferating cell nuclear antigen immunohistochemistry, respectively.

Results  At all time points after wounding, treatment with TGF-₃ significantly increased the wound breaking strength (up to 138%) and collagen deposition (up to 150%) over the control group. Cellular proliferation was increased during the first 3 weeks after wounding (up to 147%), but returned to baseline levels by the fourth week.

Conclusions  Transforming growth factor ₃ promotes fascial wound healing. In this new animal model of fascial wound healing, TGF-₃ increased fascia breaking strength, collagen deposition, and cellular proliferation. These results are similar to findings in cutaneous wound models and demonstrate, for the first time, a pharmacologic agent to accelerate fascial healing.

From the Department of Surgery, University of California, Davis–East Bay, Oakland (Dr Tyrone), Division of Plastic and Reconstructive Surgery, Northwestern University Medical School, Chicago, Ill (Drs Marcus, Mogford, Xia, and Mustoe), and Department of Surgery, Rush University Medical School, Chicago, Ill (Dr Bonomo).

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