You are seeing this message because your Web browser does not support basic Web standards. Find out more about why this message is appearing and what you can do to make your experience on this site better.


ABOUT ARCHIVES
Advanced Search

Welcome   | My Account | E-mail Alerts | Access Rights | Sign In


  Vol. 135 No. 10, October 2000 TABLE OF CONTENTS
  Archives
  •  Online Features
  Original Article
 This Article
 •Full text
 •PDF
 •Send to a friend
 • Save in My Folder
 •Save to citation manager
 •Permissions
 Citing Articles
 •Citation map
 •Citing articles on HighWire
 •Citing articles on Web of Science (16)
 •Contact me when this article is cited
 Related Content
 •Similar articles in this journal
 Topic Collections
 •Randomized Controlled Trial
 •Alert me on articles by topic
 Social Bookmarking
  Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit Add to Technorati Add to Twitter What's this?

Increased Expression of Intestinal P-Selectin and Pulmonary E-Selectin During Intravenous Total Parenteral Nutrition

Kazuhiko Fukatsu, MD; Andrew H. Lundberg, MD; M. Keith Hanna, MD; Yong Wu, MD; Henry G. Wilcox, PhD; D. Neil Granger, PhD; A. Osama Gaber, MD; Kenneth A. Kudsk, MD

Arch Surg. 2000;135:1177-1182.

Hypothesis  Intravenous total parenteral nutrition (TPN) induces intestinal polymorphonuclear neutrophil recruitment with increased intestinal intercellular adhesion molecule-1 expression. While intercellular adhesion molecule-1 causes firm adhesion of leukocytes to the endothelial cells, P- and E-selectin mediate leukocyte recruitment via rolling. Therefore, manipulation of nutrition may also affect P- and E-selectin expression in organs.

Design  Prospective randomized experimental trials.

Setting  Laboratory.

Materials  Male mice.

Interventions  Fifty-three mice were randomized to chow, intravenous TPN, or intragastric TPN.

Main Outcome Measures  After 5 days of diet, mice were administered iodine 125–labeled anti–P-selectin antibody (or iodine 125–labeled anti–E-selectin antibody) and iodine 131–labeled nonbinding antibody to quantify P-selectin (or E-selectin) expression in organs (lung, liver, kidney, small intestine, colon, stomach, pancreas, mesentery, heart, and skeletal muscle).

Results  P-selectin in small intestine, colon, stomach, and pancreas in the intravenous TPN group increased significantly as compared with the chow and the intragastric TPN groups. E-selectin expression was up-regulated after intravenous TPN in the lung but not in other sites.

Conclusions  In a time frame (5 days) when intercellular adhesion molecule-1 expression and neutrophil recruitment are increased, intestinal expression of P-selectin remains up-regulated. Early lung inflammatory changes are reflected by increases in E-selectin. This change may reflect early pulmonary dysfunction with intravenous TPN, but its significance requires further study.


From the Departments of Surgery (Drs Fukatsu, Lundberg, Hanna, Wu, Gaber, and Kudsk) and Pharmacology (Dr Wilcox), University of Tennessee, Memphis; and Department of Physiology and Biophysics, Louisiana State University Medical Center, Shreveport (Dr Granger).



Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

The Physiologic Response and Associated Clinical Benefits From Provision of Early Enteral Nutrition
McClave and Heyland
Nutr Clin Pract 2009;24:305-315.
ABSTRACT | FULL TEXT  

Intestinal Polymeric Immunoglobulin Receptor Is Affected by Type and Route of Nutrition
Sano et al.
JPEN J Parenter Enteral Nutr 2007;31:351-357.
ABSTRACT | FULL TEXT  





HOME | CURRENT ISSUE | PAST ISSUES | TOPIC COLLECTIONS | CME | SUBMIT | SUBSCRIBE | HELP
CONDITIONS OF USE | PRIVACY POLICY | CONTACT US | SITE MAP
 
© 2000 American Medical Association. All Rights Reserved.