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G Protein  7 Expression as a New Clinicopathological Marker in Patients With Intrahepatic Cholangiocarcinoma
Tohru Utsunomiya, MD;
Hiroshi Inoue, MD;
Ken-Ichi Taguchi, MD;
Mitsuo Shimada, MD;
Keizo Sugimachi, MD;
Masaki Mori, MD
Arch Surg. 2002;137:181-185.
Hypothesis The signal alterations mediated by small G proteins such as Ras, Rho, and Rac have been reported in several cancers. The human G protein  7 (G-7) gene, which is down-regulated in various digestive organ cancers, was recently identified and cloned. Thus, the G-7–coupled heterotrimeric G proteins may also contribute to carcinogenesis in human cancers.
Setting University hospital and medical institute of bioregulation.
Patients and Methods The clinicopathological significance of G-7 expression in 18 patients with intrahepatic cholangiocarcinoma (IHCC) was examined. The tumor-nontumor ratio of G-7 expression was determined using reverse transcription polymerase chain reaction analysis. To visualize the localization of G-7, an immunohistochemical study was performed.
Main Outcome Measure Clinicopathological significance of G-7 expression in human IHCC.
Results Expression of G-7 messenger RNA was lower in tumor tissue than in the corresponding nontumor tissue in 17 (94%) of 18 patients with IHCC. The mean tumor-nontumor ratio was 0.54. Eleven patients with tumor-nontumor ratios less than 0.5 showed significantly poorer differentiated IHCC than 7 with tumor-nontumor ratios of 0.5 and greater (P<.01). Decreased expression of G-7 protein in the carcinoma tissue, especially in the poorly differentiated IHCC tissue, was confirmed using immunohistochemical staining.
Conclusions Reduced expression of G-7 is associated with the histological grade of IHCC and may therefore prove to be a useful marker for predicting the biological aggressiveness of human IHCC.
From the Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan (Drs Utsunomiya, Inoue, and Mori); and the Departments of Anatomic Pathology (Dr Taguchi) and Surgery and Science (Drs Shimada and Sugimachi), Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
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