This Article  • Full text  • PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on ISI (1)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Topic Collections  • Radiation Therapy  • Alert me on articles by topic  Social Bookmarking   What's this?

Mehmet Guler, MD; Murat Kologlu, MD; Nuri Aydin Kama, MD; Nurten Renda, MD; Ugur Gozalan, MD; Yunus Nadi Yuksek, MD; Gul Daglar, MD

Arch Surg. 2002;137:813-817.

Hypothesis  Various forms of electrical stimulation can improve wound healing in different tissues, but their application to gastrointestinal tract healing has not been investigated. We assumed that positively charged diethylaminoethyl cross-linked dextran bead (diethylaminoethyl Sephadex [DEAE-S]) particles would have a beneficial effect on the healing of colonic anastomoses.

Design  Experimental animal study.

Setting  Animal research laboratory of a university hospital.

Animals  Forty female Wistar albino rats.

Interventions  Right colonic transection and anastomosis was performed in 5 animal groups. The control group received no treatment; the placebo group, methylcellulose gel; and the DEAE-S group, DEAE-S in methyl cellulose gel applied topically around the anastomoses. The fecal peritonitis (FP) group underwent cecal ligation and perforation simultaneously with the anastomosis to cause FP; the FP + DEAE-S group also received DEAE-S applied around the anastomoses.

Main Outcome Measures  After the completion of postoperative day 4, all rats were killed. Anastomotic bursting pressures and hydroxyproline concentrations in perianastomotic tissue were measured and compared.

Results  Mean bursting pressures were 115.1 mm Hg in the control group, 113.6 mm Hg in the placebo group, 159.4 mm Hg in the DEAE-S group, 62.8 mm Hg in the FP group, and 121.1 mm Hg in the FP + DEAE-S group (P = .001, 1-way analysis of variance [ANOVA]). The differences between the control vs DEAE-S groups, placebo vs DEAE-S groups, and FP vs FP + DEAE-S groups were significant (P<.05, t test). Mean hydroxyproline concentrations were 5.2 µg/mg in the control group, 4.9 µg/mg in the placebo group, 5.6 µg/mg in the DEAE-S group, 4.5 µg/mg in the FP group, and 5.4 µg/mg in the FP + DEAE-S group (P = .09, 1-way ANOVA). The difference between the FP and FP + DEAE-S groups was significant (P = .04, t test).

Conclusions  A positively charged particle, DEAE-S, improves healing of colonic anastomoses in healthy rats and in rats with FP. This inexpensive, nontoxic material is easily applied and deserves further evaluation in gastrointestinal tract healing.

From the Fourth Department of Surgery, Ankara Numune Hospital (Drs Guler, Kologlu, Kama, Gozalan, Yuksek, and Daglar), and the Department of Biochemistry, Hacettepe University (Dr Renda), Ankara, Turkey.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati     What's this?