This Article  • Full text  • PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Citing articles on ISI (8)  • Contact me when this article is cited  Related Content  • Related article  • Similar articles in this journal  Topic Collections  • Surgical Physiology, Other  • Gastrointestinal Diseases  • Genetics  • Genetic Counseling/ Testing/ Therapy  • Alert me on articles by topic  Social Bookmarking   What's this?

Bruce Hermann, MD; Yan Li, PhD; Mukunda B. Ray, MD; John M. Wo, MD; Robert C. G. Martin II, MD

Arch Surg. 2005;140:1204-1209.

Hypothesis  The down-regulation of manganese superoxide dismutase (MnSOD) expression plays a role in the progression of Barrett esophagus (BE).

Design  An evaluation of 92 esophageal samples, including 17 patients with normal esophagus, 22 with intestinal metaplasia, 22 with indefinite/low-grade dysplasia, 16 with high-grade dysplasia (HGD), and 15 with esophageal adenocarcinoma were evaluated for MnSOD expression. We evaluated MnSOD expression using immunohistochemistry and graded it separately on a 2-category ordinal scale in relation to the mucosa and submucosa that ranged from 0 (no staining) to 3 (strong staining). The total grading score of MnSOD immunoreactivity was the addition of mucosa and submucosa intensity, from 0 (no immunoreactivity in any of the anatomic sites) to a maximum score of 6 (strong staining reaction in both of the histoanatomic sites).

Setting  Study subjects were recruited from the Barrett’s Esophageal Registry at the University of Louisville, Louisville, Ky.

Main Outcome Measure  Manganese superoxide dismutase expression in established groups of progressive BE.

Results  Ninety-two samples were evaluated for MnSOD expression. The expression of MnSOD was found to be significantly reduced in samples with specialized intestinal metaplasia (mean score, 1.8), low-grade dysplasia (mean, 2.2), high-grade dysplasia (mean, 2.4), and esophageal adenocarcinoma (mean, 2.4) when compared with normal esophagus (mean, 3.9; P = .002). Manganese superoxide dismutase expression was similar for esophageal adenocarcinoma and high-grade dysplasia.

Conclusions  Manganese superoxide dismutase expression is significantly reduced in patients with BE with high-grade dysplasia and esophageal adenocarcinoma. Manganese superoxide dismutase is related to the progression of BE and may represent one of the primary factors in oxidative stress protection. Further evaluation within genotypic expression and the role of antioxidants is needed in the effective screening and treatment of BE.

Author Affiliations: Department of Surgery, Division of Surgical Oncology (Drs Hermann, Li, and Martin), Department of Pathology (Dr Ray), and Department of Medicine, Division of Gastroenterology/Hepatology (Dr Wo), University of Louisville School of Medicine, Louisville, Ky.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati     What's this?

RELATED ARTICLE

Association of Manganese Superoxide Dismutase Expression With Progression of Carcinogenesis in Barrett Esophagus—Invited Critique
John W. Harmon, Pramod Bonde, and Chiming Wei
Arch Surg. 2005;140(12):1209.
EXTRACT | FULL TEXT  

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Manganese Superoxide Dismutase (MnSOD) Polymorphism, Alcohol, Cigarette Smoking and Risk of Oesophageal Cancer
Sun et al.
Alcohol Alcohol 2009;44:353-357.
ABSTRACT | FULL TEXT  

Review: Chemoprevention of esophageal adenocarcinoma
Abrams
Therapeutic Advances in Gastroenterology 2008;1:7-18.
ABSTRACT  

Alterations in Manganese Superoxide Dismutase Expression in the Progression From Reflux Esophagitis to Esophageal Adenocarcinoma
Li et al.
Ann. Surg. Oncol. 2007;14:2045-2055.
ABSTRACT | FULL TEXT